Journal article Open Access

Determining the fraction of cells with stem-like properties in the HSJD DIPG 007 cell line by limiting dilution assay

Brown, Elizabeth J; Farnie, Gillian; Bullock, Alex N

A description of how an limiting dilution assay was developed to test the stem-cell-like compartment within the HSJD-DIPG-007 patient derived cell line.

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
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