Dataset Open Access

3dpf zebrafish larvae, 96 well plate,Tg(wt1b:EGFP), dorsal view, ACQUIFER Imaging Machine

Gehrig, Jochen

Datasets originate from internal test runs at ACQUIFER on the Imaging Machine (see also application note on "The ACQUIFER PlateViewer: A tool for visualizing high content screening data and supervised feedback microscopy")

The datasets contain images acquired from 96 Tg(wt1b:EGFP) embryos at 3 dpf aligned in agarose cavities generated with 3d printed orientation tools (Wittbrodt, Jonas N., Urban Liebel, and Jochen Gehrig. "Generation of orientation tools for automated zebrafish screening assays using desktop 3D printing." BMC biotechnology 14.1 (2014): 36.):

- 2x, 4x, 10x views

- for each well (e.g. A001) a multi-color z-stack was acquired, with  N z-slices (SLNNN) in two channels: BF (CO6) and GFP (CO3)

Example file name containing metadata: -A001--PO01--LO001--CO3--SL001--PX32500--PW0100--IN0100--TM281--X014580--Y011262--Z210710--T0200256066--WE00001.tif

Used also as benchmark dataset for Multi-Template Matching by Thomas, LSV and Gehrig, J

See implementation in Fiji https://github.com/LauLauThom/MultipleTemplateMatching

and in KNIME https://github.com/LauLauThom/MultipleTemplateMatching-KNIME

Contact:

j.gehrig(at)acquifer.de, l.thomas(at)acquifer.de

 

Files (47.5 GB)
Name Size
wt1b-dorsal-10x.zip
md5:ef04ee66f02d615f28a41955efab536f
31.8 GB Download
wt1b-dorsal-2x.zip
md5:533f0055ca9718f6fdf3578d1a599224
4.6 GB Download
wt1b-dorsal-4x.zip
md5:c7b1ac52a182aa785397e8c1ba3e02c4
11.1 GB Download
31
6
views
downloads
All versions This version
Views 3131
Downloads 66
Data volume 88.6 GB88.6 GB
Unique views 2323
Unique downloads 55

Share

Cite as