Global Animal Science Journal
Published July 1, 2014 | Version v1
Journal article Open

Genetic detection of IB and other respiratory viral diseases in chicken flocks in Egypt

Authors/Creators

  • 1. Virology department, faculty of Veterinary medicine, Suez Canal University. Egypt
  • 2. Central Laboratory for Evaluation of Veterinary Biologics, Abbassia, Cairo, Egypt

Description

Newcastle  disease  (ND),  infectious  bronchitis  (IB)  and  avian  influenza  (AI)  are  highly  contagious and  are  responsible  for  huge  economic  losses  in  poultry  production. Thirteen chicken flocks showed upper respiratory tract manifestation from Ismailia, Sharkia and Dumyat provinces during 2011 and 2012 winter seasons. SPF embryonated chicken eggs were used for virus isolation. Results of HI and PCR indicated that, 13 isolates were positive to IBV and negative for AIV. In addition, 6 isolates were positive to NDV. Six chicken flocks had mixed infection with IBV and NDV, while seven flocks were infected with IBV only. The PCR of IBV targeted ORF1a gene of IBV while targeting the F gene of NDV.

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