Large-scale purification of HTT domain constructs 2019/04/01
Authors/Creators
- 1. Structural Genomics Consortium, University of Toronto
- 2. Structural Genomics Consortium, University of Toronto, MaRS South Tower, 101 College Street, Toronto, Ontario M5G 1L7, Canada
- 3. CHDI Foundation
Description
Project: High resolution structural analysis of purified HTT samples
Experiment: Large-scale purification of HTT domain constructs
Date completed: 2019/04/01
Rationale: Domain fragments of the huntingtin protein are useful reagents for examining protein-protein interaction characteristics of huntingtin and to map interaction interfaces. Stable, monodisperse and pure samples may also be amenable to high resolution structure solution by X-ray crystallography. Previously huntingtin fragments were cloned and screened for expression in small-scale (3 mL) culture experiments. Positive hits were scaled for production as detailed in the table on the next page. All previous work is described in this post:https://zenodo.org/record/2600051#.XKU89aeZPOQ.