Raw diffraction images of Ferroportin homologue (phasing data by Hg-SIRAS)

Diffraction images of native and mercury-bound Ferroportin homologue from Bdellovibrio bacteriovorus. This phase information was used for PDB entries 5AYM and 5AYN, and indirectly 5AYO (Taniguchi et al. 2015). All data were collected from loop-harvested crystals on BL32XU, SPring-8 at a wavelength of 1.00000 Å using the MX225HS CCD detector.

From a native crystal, a helical (180°/crystal, 1°/frame) datasets were collected using 12×8 μm² beam. From a mercury-derivative crystals, two helical (180°/crystal, 1.5°/frame) datasets were collected using 10×2 μm² beam. The crystals belonged to space group P2₁2₁2₁ with unit cell parameter a~57, b~86, c~96 Å.