Macromolecular Crystallography
Published July 12, 2013 | Version v1
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Raw diffraction images of H+/Ca2+ exchanger CAX (phasing data by Hg-MAD)

Authors/Creators

  • 1. Graduate School of Science, The University of Tokyo
  • 2. RIKEN SPring-8 Center

Description

Diffraction images of mercury-bound H+/Ca2+ exchanger from Archaeoglobus fulgidus. This phase information was used for a PDB entry 4KPP (Nishizawa et al. 2013). All data were collected from loop-harvested crystals on BL32XU, SPring-8 at wavelengths of 1.00000 Å (peak), 1.00930 Å (edge), or 1.01300 Å (low remote) using the MX225HE CCD detector.

From a mercury-derivatised crystal, helical (360°/crystal, 1°/frame) datasets were collected using 5×1 μm2 beam. The crystal belonged to space group P21 with unit cell parameter a~64, b~98, c~72 Å, β~99°.

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Additional details

Related works

Cites
10.1126/science.1239002 (DOI)