Journal article Open Access
Onyeto C. A; Ihim S. A.; Akah P. A.
The hepatoprotective effect of methanol leaf extract (ME) of Lophiralanceolata Tiegh (Ochnaceae) was investigated against carbon tetrachloride (CCI4) induced hepatotoxicity in rats. Rats were randomly divided into 7 groups(n=6) and ME were administered at 100, 200, and 400mg/kg/day p.o to groups III-V, while groups I, VI and VII received 1ml/kg tween 80, Vitamin C and Silymarin at 25 mg/kg/day p.o respectively while group II received only CCl4.. CCI4 was given every 72 hours to all the groups except group1.ThenSerum level of blood samples was assayed by alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), malondialdehyde (MDA), total protein, total bilirubin, albumin and globulin. ME significantly (p < 0.05) reduced in the serum levels of the liver enzymes when compared with the control group (CCI4). The levels of ALT, AST and bilirubin at 100 and 400mg/kg were reduced significantly (p< 0.05).Also, ALP levels was lowered at 100 mg/kg but not significant difference (p>0.05). At 100mg/kg, MDA levels was lowered more significantly (p<0.05) than controls (Silymarin and vitamin C).Serum globulin level at 200mg/kg dose of extract was significantly increased when compared to CCI4 control group.
In conclusion, ME can be protective against oxidative stress caused by liver toxicitylike CCl4.