Published December 3, 2018 | Version v1
Dataset Open

Multi-angle light scattering (MALS) analysis of HTT1-3144 Q54 HAP401-371 samples purified from Sf9 insect cells - 2018/12/03

Authors/Creators

  • 1. Structural Genomics Consortium, University of Toronto
  • 2. OICR
  • 3. CHDI Foundation

Description

Project: Biophysical investigation of purified HTT protein samples

Experiment: Multi-angle light scattering (MALS) analysis of HTT1-3144 Q54 HAP401-371samples purified from Sf9 insect cells

Date completed:­ 2018/12/03

Rationale: Apo HTT1-3144 Q23 and HTT1-3144 Q23 HAP401-371complex samples have been analysed by MALS revealing HTT-HAP40 complex samples to be monodisperse. PolyQ expanded HTT constructs have been expressed as HAP40 complexes but the monodispersity has not been assessed beyond analytical gel filtration. 

Notes

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

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Multi-angle light scattering (MALS) analysis of HTT1-3144 Q54 HAP401-371 samples purified from Sf9 insect cells 20191203.pdf

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