Effect of NAA and BAP on In-Vitro Micropropagation of Vitex negundo L. - A Significant Medicinal Plant
Authors/Creators
- 1. Department of Botany, Government M.V.M. Bhopal, Madhya Pradesh (India)
- 2. Department of Botany, Rajeev Gandhi College, Shahpura, Bhopal, Madhya Pradesh (India)
Description
Vitex negundo has been used worldwide in pharmaceutical industry. Its natural vegetative propagation rate is unable to meet growing demands of pharmaceutical industries. The present study is aimed to find a highly effective and a rapid method for the Micropropagation of this medicinal plant to meet the demand. The explants of V. negundo were cultured on Murashige and Skoogs (MS) medium supplemented with different concentrations of auxins, cytokinins and sucrose. Among different combinations used, Murashige and Skoog medium with 0.5 (mg L–1) 6-benzylaminopurine + 0.5 (mg L–1) α-naphthalene acetic acid, containing 0.8% agar and 25% sugarcane juice exhibited highest shoot induction (80%) with highest number of shoots per explant (3 - 4) supplemented with 3% Sucrose and 0.8% (w/v) agar within 20 days.