EVOLUTIONOF NEMATICIDAL POTENTIAL OF CRUDE EXTRACT OF MEDICINAL PLANTS FROMPAKISTAN FLORA

Root knot nematodes are harming various plants which is economically harmful and can lead towards serious food and economics problem. The research was carried out with aim to point out nematicidal potential of Methanolic extract of five selected medicinal plants, Chinopodium album, Withania Coagulans, Saphora mollis, Peganum harmala and Rosamarinus officinalis. Methanolic extract were used for extract preparation. 80% Methanolic solvent was used. All extracts were prepared in SBKWU lab and nematicidal analysisof crude extract of medicinal plants was carried out in NNRC (national nematicidal research center) Karachi. Culture for incognita species obtained from Lycopersicon esculentum (tomato) plant were prepared at 28 ⁰C incubation temperature. For nematicidal assay, second javelined stage was utilized.  The analysis was carried out with different dose including 1%, 0.5%, 0.25% and 0.125%. Analysis was made with duration of 0.5, 1, 2, 3, 4, 42, 48 and 72 hours. Significant response was observed in Chinopodium album whose response was 100% reduction in nematodes population at 48 and 72 hours of exposure. All dose of this plant extract also showed positive response but among five selected herbal plants, lowest response was found in Saphora mollisMethanolic extract with 21-51.5 % response at even maximum exposure with highest dose.

Key Words: Root-Knot Nematodes, Nematicidal potential, Methanolic Extract, Medicinal Plants, MeloiogyneSpecies.