ISOLATION OF MDR MICROORGANISMS FROM NATURAL SOURCES, STUDYING THEIR PLASMID PROFILE AND EVALUATION OF POTENCY FOR NATURAL TRANSFER OF MDR PROPERTY TO E. COLI DH5Α.

The water samples from various sites on the banks of Pavanariver, Pune were screened for presence of pathogenic Salmonella, Shigella, Pseudomonas, E. coli, Streptococcus and Staphylococcus. Some of the isolated pathogens showed resistance to ampicillin, streptomycin and erythromycin. To understand antibiotic resistance is Plasmid DNA borne, isolation of plasmid was carried out by alkaline lysis method, plasmid profile was analyzed by agarose gel electrophoresis and plasmids were cured from the isolates by growing them in nutrient broth containing 100?g/ml ethidium bromide. Property of antibiotic resistance from MDR isolates was transferred to E. coli DH5α by growingE. coli DH5α with cell free extract of MDR isolates.