Dataset Open Access

Data for: Optogenetic olfactory behavior depends on illumination characteristics

Tumkaya, Tayfun; Stewart, James; Burhanudin, Safwan B.; Claridge-Chang, Adam

Abstract

Optogenetics has become an important tool to study behavior: it enables neuroscientists to infer causations by examining animal behavior after activating genetically circumscribed neurons with light. Light-induced activity is profoundly affected by illumination parameters used in experiments, such as intensity, duration, and frequency. How sensitive behavioral outcomes to light-dependent spike changes has not been extensively studied. Here, we investigated the hypothesis that light frequency would alter optogenetically induced behaviours. To test this, we activated olfactory receptor neurons (ORNs) in Drosophila by using either static- or pulsed-light stimuli. Static- and pulsed-light stimulations elicited distinct valence responses (attraction, aversion, neutral) to artificial activity in ORNs. Our results demonstrate the importance of light frequency for interpreting behavioral experiments accurately, and suggest that multiple light parameters should be tested before generalizing behavioral experiment results.

Files (2.7 GB)
Name Size
Data_and_Figures.rar
md5:e7a463a8bcd6e7c195b6fedbe545154b
2.7 GB Download
49
9
views
downloads
All versions This version
Views 4949
Downloads 98
Data volume 24.4 GB21.7 GB
Unique views 3636
Unique downloads 87

Share

Cite as