Journal article Open Access
Dr.VamseeKrishna. G*1, Dr.Y.A. Chowdary1, S.N.S. Sriram1, P. Lavanya2, G. Varsha Sri2, Sk.Sujauddin2
Rizatriptan Benzoate is a selective 5-Hydroxy Tryptamine 1B/1D receptor agonist, which is used in the treatment of migraine headache. Three simple, sensitive, selective, accurate, precise, economical, robust, rapid and reproducible ultraviolet (UV) spectrophotometric methods have been developed and validated for quantitative estimation of Rizatriptan benzoate in bulk and tablet dosage form. The solutions of Rizatriptan were prepared using water and solvent blends containing acetonitrile: H2O (1:1), water: methanol (1:1) and studied their influence on estimation of Rizatriptan benzoate. The three different solutions of drug were scanned in the UV region of 200nm-400nm. Rizatriptan benzoate showed maximum absorbance at 224nm in all three different solvents, and obeyed Beer-Lambert’s law in the concentration range of 2-12μg/ml. The linear regression equations were calculated to be Y=0.913x+0.0065 for acetonitrile: water (1:1) (R2 =0.9994), Y=0.0896x+0.0101 for methanol: water (1:1) (R2 =0.9994) and Y=0.0858x+0.0134 for water (R2 =0.9992). The analytical parameter such as linearity, A(1%/1cm), limit of detection (LOD), limit of quantification(LOQ), intermediate precision, molar absorption coefficient and sandell’s sensitivity were calculated. The validation parameters were treated statistically with one-way analysis of variance(ANOVA) and significant differences were noted. The study clearly revealed that the solvents influence the determination of Rizatriptan benzoate, the method described was rapid and easy. So it can be used for routine quality control analysis of Rizatriptan benzoate in pharmaceutical preparations.