Published August 21, 2018 | Version v1
Journal article Open

Allocation of rhodamine-loaded nanocapsules from blood circulatory system to adjacent tissues assessed in vivo by fluorescence spectroscopy

Authors/Creators

  • 1. University of Oulu, Finland; St. Petersburg Academic University, Russia; Research Institute of Pediatric Oncology, Hematology and Transplantology named by R.M. Gorbacheva, Russia
  • 2. Orеl State University, Russia
  • 3. Orеl State University, Russia; Centre of Preclinical Research, JSC "Retinoids", Russia
  • 4. Aston University, UK
  • 5. University of Oulu, Finland; National Research Tomsk State University, Russia
  • 6. University of Oulu, Finland; Orеl State University, Russia; National Research Tomsk State University, Russia; National Research Nuclear University "MEPhI", Russia

Description

Modern fluorescent modalities play an important role in the functional diagnostic of various physiological processes in living tissues. Utilizing the fluorescence spectroscopy approach we observe the circulation of fluorescent-labelled nanocapsules with rhodamine tetramethylrhodamine in a microcirculatory blood system. The measurements were conducted transcutaneously on the surface of healthy Wistar rat thighs in vivo. The administration of the preparation capsule suspension with a rhodamine concentration of 5 mg kg−1 of the animal weight resulted in a two-fold increase of fluorescence intensity relative to the baseline level. The dissemination of nanocapsules in the adjacent tissues via the circulatory system was observed and assessed quantitatively. The approach can be used for the transdermal assessment of rhodamine-loaded capsules in vivo.

Files

LPL 2018 - Allocation of rhodamine-loaded nanocapsules.pdf

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Additional details

Funding

European Commission
FORECAST - Fluorescence lifetime optical biopsy system 703145