Planned intervention: On Wednesday April 3rd 05:30 UTC Zenodo will be unavailable for up to 2-10 minutes to perform a storage cluster upgrade.
Published September 16, 2016 | Version v1
Poster Open

In vitro and in vivo investigations to obtain validated toxicity data of graphene nanoplatelets

  • 1. BIOTOX Srl, Cluj-Napoca, Romania
  • 2. Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany
  • 3. Department of Biology, CESAM &CICECO, University of Aveiro, Aveiro, Portugal

Description

The PLATOX project funded within the FP7-SIINN (Safe Implementation of Innovative Nanoscience and Nanotechnology) ERANET on Nanosafety, proposes a tiered approach to address the existing toxicological data gaps for graphene family nanoparticles (GFN). GFN are part of the group of carbonbased synthetic nanomaterials and are already on the market in multiple variants. GFN are currently subject of studies to accelerate their toxicological characterisation. Based on the existing toxicological database a very high toxic potential is not expected, however, the characterisation is presently incomplete and should be expanded to facilitate a proper risk assessment. The workflow of PLATOX therefore aims to link several steps i) selection of typical candidates, representing the graphene family, ii) performance of in vitro screening for the selected GFN, iii) conduct of an in vivo 28day inhalation test with two representative GFN, thereby generating toxicological in vivo data, which should be acceptable for authorities and should enable iv) risk assessment and derivation of DNEL for the two selected GFN according to REACH procedures. The in vitro screening methodology uses lung relevant cell models and is focussed on (geno)toxicity testing and measurement of cytokine/eicosanoid release. The subsequent 28day inhalation toxicity test will include inflammatory, (geno)toxicity and histopathological endpoints. The results will finally be evaluated and a GFN risk assessment will be done by establishing DNELs. In summary, the expected outcome of the project will be a toxicological ranking of the tested GNF species, providing an improved basis for risk assessment of these nanomaterials.

Files

PLATOX_Eurotox_poster_v2.1.pdf

Files (828.2 kB)

Name Size Download all
md5:e640ddabd107d619c59637b00ea31150
828.2 kB Preview Download

Additional details

Funding

SIINN – Safe Implementation of Innovative Nanoscience and Nanotechnology 265799
European Commission