FORMULATION AND EVALUATION OF HYPTIS SUAVEOLENS BASED BIOADHESIVE VAGINAL GELS

ABSTRACT

The aim of the present undertaken work is to formulate and evaluate vaginal gel containing Hyptis suaveolens leaf petroleum leaf extract. The prepared extract was subjected to phytochemical screening to determine the presence of various phytoconstituents.  The extract was subjected to formulation of gels incorporating gelling agents (Carbopol 934P, HPMC K100, Pemulen TR-1, Pemulen TR2, Xanthan gum, Lutrol) in various concentrations by standard gelling techniques. All the HS Gel formulations were subjected to characterization of various physical parameters like Appearance, Spreadability, Viscosity, pH and Extrudability as per the standard methods. pH values of various HS Gel formulations were found to be unsuitable, as the desired vaginal pH is between 4.5 and 5.5. Owing to the inferior results of physical parameters, which were not considered for further characterization. CF3, HF3, P1F3, P2F3, LF3 XF3 Gel formulations of Hyptis suaveolens consisting high concentrations of respective gelling agents have shown encouraging results with respect to physical parameters. These Gel formulations of Hyptis suaveolens consisting high concentrations of respective gelling agents were subjected to in vitro diffusion studies. By comparing the results of in vitro diffusion studies, Hyptis suaveolens Gel formulations consisting Carbopol 934P (CF3) was optimized due to its retarding ability and consistency. In vivo studies of the optimized vaginal gel formulations were carried out using rats as the animal model to evaluate the antifertilty activity. Results of Antifertility studies indicated that the petroleum ether extract from the leaves of Hyptis suaveolens was found to be efficient to arrest sperm motility and conceiving.

KEYWORDS: Hyptis suaveolens, Carbopol 934P, vaginal gel, Xanthan gum, Antifertility.