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Published December 11, 2018 | Version v1.0.0
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Comparison of re-called Albacore and Flappie sequences from E. coli K-12 MG1655

Authors/Creators

  • 1. Gringene Bioinformatics

Description

I used the first few (545) original Fast5 files from Nick Loman's ultra-long read E. coli K-12 MG1655 R9.4 sequencing run. See the blog post here. Direct link to the complete fast5 dataset here.

Program versions:

* Albacore 2.1.10

* Flappie 1.0.0-0048dfd

The example alignment was carried out using seaview (1:4.6.1.2-2), and visualised using spiralign from my bioinfscripts repository (see source code in this archive):

    $ spiralign.r -size 2000x2000 -noalign -noborder -loops 12.75 -outfmt png -type nucl -title "Flappie vs Albacore\n(Ecoli_MG1655)" aligned_all_ddea.fa

Files

msa_nucl.png

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Additional details

Related works

Cites
http://lab.loman.net/2017/03/09/ultrareads-for-nanopore/ (URL)