Dataset Open Access

Extracellular recordings from the locust, Schistocerca americana, olfactory pathway

Pouzat, Christophe; Mazor, Ofer; Laurent, Gilles

This depository contains raw data from 14 experiments performed on adult locusts. The data are contained in HDF5 files (http://www.hdfgroup.org/HDF5/). They are stored as compressed integers coded on 16 bits as they came out of the A/D card. Recording details can be found in Pouzat, Mazor and Laurent (2002) Using noise signature to optimize spike-sorting and to assess neuronal classification quality. Journal of Neuroscience Methods 122: 43-57 (a pre-print version is available: http://xtof.perso.math.cnrs.fr/pdf/Pouzat+:2002.pdf). Each data file is subdivided in Groups corresponding the type of acquisition performed: one or several epochs of spontaneous activity recording; repetitive stimulation with a given odor. Each group is made of one (if say a single epoch of 60 seconds of spontaneous recording was made) or several (if say 100 stimulation with Citral were made) (sub-)groups containing the data of all the channels that were recorded during that epoch. Each of these sub-groups is made of 4 to 16 data sets: 1 dimensional arrays containing the raw data recorded from one of the 16 channels of our probe (made of 4 tetrodes) during a single acquisition epoch. All channels were sampled at 15 kHz. Each data file has attributes (metadata) README and LabBook. The first, README contains a shortened version of the present text; the second, LabBook contains a transcript of the lab book corresponding to the experiment. Most groups have a log_file_content attribute. This attribute contains a copy a text file that was automatically generated during data acquisition. Some recording details can be found there as well as the precise time and data of each recorded epoch. The data were kept for 14 years on CDs and about a third of the recordings got lost because of CD corruption! What's left still make 15 GBytes of data after compression: a substantial amount. This CD corruption explains why some groups don't have a log_file_content attribute: it was on a corrupted CD.

Of the 14 experiments, 12 contain antennal lobe (the first olfactory relay of insects) recordings, 1 contains antennal lobe and alpha lobe recordings and 1 contains only alpha lobe recordings. Here the alpha lobe location should be taken with a little bit of caution since the latter is not as easy to locate as the antennal lobe in the locust. All data files start with the locust prefix, followed by the experiment year, month and date, like locust20000214.hdf5 an experiment performed on February 14 2000. Due to file size restriction on Zenodo, two experiments are split into two data files: locust20010124b_part1.hdf5 and locust20010124b_part2.hdf5 as well as locust20010214_part1.hdf5 and locust20010214_part2.hdf5. On two dates, two different experiments were performed: locust20010124a.hdf5 and locust20010124b_part1.hdf5 / locust20010124b_part2.hdf5 as well as locust20010208a.hdf5 and locust20010208b.hdf5. When a stimulation was applied, the following code is used: Odor name / Number of stimulation / Inter-stimulation interval / duration before the odor pulse / odor pulse duration / post pulse duration [odor dilution when several dilutions were used]. All times are in seconds.

A very brief description at the group level of the files content follows (see the LabBook attribute of each individual file for details):

  1. locust20000214.hdf5:
    • Citral / 70 / 30 / 3 / 0.5 / 6.5
    • Cherry / 120 / 30 / 3 / 0.5 / 6.5
    • Octaldehyde / 60 / 30 / 3 / 0.5 / 6.5
  2. locust20000421.hdf5:
    • Spontaneous: 60 seconds of spontaneous activity
    • 1-Hexanol / 30 / 10 / 3 / 1 / 5.5
    • Hexanal / 25 / 10 / 3 / 1 / 5.5
    • Cis-3-hexen-1-ol / 25 / 10 / 3 / 1 / 5.5
    • Trans-2-hexen-1-ol / 25 / 10 / 3 / 1 / 5.5
    • 1-Hexen-3-ol / 25 / 10 / 3 / 1 / 5.5
    • 3-Pentanone / 25 / 10 / 3 / 1 / 5.5
    • 1-Heptanol / 25 / 10 / 3 / 1 / 5.5
    • 1-Octanol / 25 / 10 / 3 / 1 / 5.5
    • 2-Heptanone / 25 / 10 / 3 / 1 / 5.5
    • 3-Heptanone / 25 / 10 / 3 / 1 / 5.5
    • Citral / 25 / 10 / 3 / 1 / 5.5
    • Apple / 25 / 10 / 3 / 1 / 5.5
    • Mint / 25 / 10 / 3 / 1 / 5.5
    • Strawberry / 25 / 10 / 3 / 1 / 5.5
    • Octaldehyde / 25 / 10 / 3 / 1 / 5.5
    • 1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-5]
    • 1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-4]
    • 1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-3]
    • 1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-2]
    • 1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-1]
    • 1-Octanol / 25 / 10 / 3 / 1 / 5.5 [1]
  3. locust20000423.hdf5:
    • Spontaneous first: 60 seconds of spontaneous activity
    • 1-Hexanol / 25 / 10 / 3 / 1 / 5.5
    • Hexanal / 25 / 10 / 3 / 1 / 5.5
    • Cis-3-hexen-1-ol / 25 / 10 / 3 / 1 / 5.5
    • 1-Hexen-3-ol / 25 / 10 / 3 / 1 / 5.5
    • 1-Heptanol / 25 / 10 / 3 / 1 / 5.5
    • 2-Heptanone / 25 / 10 / 3 / 1 / 5.5
    • 3-Heptanone / 25 / 10 / 3 / 1 / 5.5
    • Citral / 25 / 10 / 3 / 1 / 5.5
    • Apple / 25 / 10 / 3 / 1 / 5.5
    • Amyl Acetate / 25 / 10 / 3 / 1 / 5.5
    • 1-Hexanol / 25 / 10 / 3 / 1 / 5.5
    • Spontaneous second: 60 seconds of spontaneous activity
  4. locust20000613.hdf5:
    • Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1]
    • Cis-3-hexen-1-ol / 10 / 30 / 3 / 1 / 16 [1/100]
    • Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1/10]
    • Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1]
    • Cherry / 21 / 30 / 3 / 1 / 16
  5. locust20000616.hdf5:
    • Spontaneous first: 60 seconds of spontaneous activity
    • Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1]
    • Spontaneous second: 60 seconds of spontaneous activity
    • Spontaneous third: 60 seconds of spontaneous activity
    • Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1/100]
    • Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1/10]
  6. locust20000901.hdf5:
    • Vanilla / 5 / 30 / 3 / 1 / 16
    • Spontaneous: 60 seconds of spontaneous activity
    • Cherry / 30 / 30 / 3 / 1 / 16
    • Spontaneous: 60 seconds of spontaneous activity
    • Benzaldehyde / 30 / 30 / 3 / 1 / 16
    • Spontaneous: 60 seconds of spontaneous activity
    • Mint / 20 / 30 / 3 / 1 / 16
    • Hexanal / 15 / 30 / 3 / 1 / 16
    • Spontaneous: 60 seconds of spontaneous activity
    • Cis-3-hexen-1-ol / 30 / 30 / 3 / 1 / 16
    • Spontaneous: 60 seconds of spontaneous activity
    • Trans-2-hexen-1-ol / 30 / 30 / 3 / 1 / 16
  7. locust20010124a.hdf5:
    • Spontaneous: 2x29 seconds of spontaneous activity
    • Spontaneous: 60x29 seconds of spontaneous activity
    • Spontaneous: 80x29 seconds of spontaneous activity
  8. locust20010124b_part1.hdf5 and locust20010124b_part2.hdf5:
    • Spontaneous: 60x29 seconds of spontaneous activity
    • Spontaneous: 191x29 seconds of spontaneous activity
    • Spontaneous: 59x29 seconds of spontaneous activity
  9. locust20010131.hdf5:
    • Spontaneous: 90x29 seconds of spontaneous activity
    • Spontaneous: 70x29 seconds of spontaneous activity
    • Spontaneous: 5x59 seconds of spontaneous activity
    • Spontaneous: 3x59 seconds of spontaneous activity
    • Spontaneous: 3x59 seconds of spontaneous activity
    • Spontaneous: 3x59 seconds of spontaneous activity
    • Spontaneous: 3x59 seconds of spontaneous activity
    • Spontaneous: 3x59 seconds of spontaneous activity
    • Spontaneous: 3x59 seconds of spontaneous activity
    • WithoutAntenna: 3x59 seconds of spontaneous activity (after antennal nerve cut)
    • WithoutAntenna: 3x59 seconds of spontaneous activity (after antennal nerve cut)
    • WithoutAntenna: 3x59 seconds of spontaneous activity (after antennal nerve cut)
  10. locust20010201:
    • Continuous: 90x29 seconds of spontaneous activity
    • Continuous: 20x29 seconds of spontaneous activity
    • Citral / 50 / 30 / 3 / 1 / 25
    • Citral / 50 / 30 / 10 / 1 / 18
    • Citral / 50 / 30 / 10 / 1 / 18
    • Continuous: 50x29 seconds of spontaneous activity
    • Continuous: 45x29 seconds of spontaneous activity
  11. locust20010208a.hdf5:
    • Spontaneous: 50x29 seconds of spontaneous activity
    • Spontaneous: 80x29 seconds of spontaneous activity
  12. locust20010208b.hdf5:
    • Spontaneous: 50x29 seconds of spontaneous activity
    • Spontaneous: 50x29 seconds of spontaneous activity
    • Citral / 50 / 30 / 10 / 1 / 18
    • Citral / 120 / 30 / 10 / 1 / 18
    • Citral / 50 / 30 / 10 / 1 / 18
    • Citral / 25 / 30 / 10 / 1 / 18
  13. locust20010214_part1.hdf5 and locust20010214_part2.hdf5:
    • Spontaneous: 30x29 seconds of spontaneous activity
    • Spontaneous: 30x29 seconds of spontaneous activity
    • Cis-3-hexen-1-ol / 25 / 30 / 10 / 1 / 18
    • Citral / 25 / 30 / 10 / 1 / 18
    • Vanilla / 25 / 30 / 10 / 1 / 18
    • Octanol / 25 / 30 / 10 / 1 / 18
    • Mint / 25 / 30 / 10 / 1 / 18
    • Cis-3-hexen-1-ol / 25 / 30 / 10 / 1 / 18
    • Spontaneous: 30x29 seconds of spontaneous activity
    • Spontaneous: 30x29 seconds of spontaneous activity
    • Cis-3-hexen-1-ol / 30 / 30 / 10 / 1 / 18
    • Cis-3-hexen-1-ol / 11 / 30 / 10 / 1 / 18
    • Cis-3-hexen-1-ol / 30 / 30 / 10 / 1 / 18
    • Cis-3-hexen-1-ol / 30 / 30 / 10 / 1 / 18
  14. locust20010217.hdf5:
    • Spontaneous: 10x29 seconds of spontaneous activity
    • Spontaneous: 2x29 seconds of spontaneous activity
    • Spontaneous: 30x29 seconds of spontaneous activity
    • Spontaneous: 10x29 seconds of spontaneous activity
    • Spontaneous: 10x29 seconds of spontaneous activity
    • Spontaneous: 10x29 seconds of spontaneous activity
    • Spontaneous: 10x29 seconds of spontaneous activity
    • Spontaneous: 10x29 seconds of spontaneous activity
    • Spontaneous: 10x29 seconds of spontaneous activity

Files (13.4 GB)
Name Size
locust20000214.hdf5
md5:1c9401a6524cb90bd027880c0bd35f48
334.0 MB Download
locust20000421.hdf5
md5:7bd67fb701ec7365b325bbc7bb9f4887
841.3 MB Download
locust20000423.hdf5
md5:d0b3842464d4202b98f794ec38aca128
433.9 MB Download
locust20000613.hdf5
md5:c98d3defb265175efe8236726938efa6
858.5 MB Download
locust20000616.hdf5
md5:d53b134b5eb7a1cc6acedd794c974614
453.1 MB Download
locust20000901.hdf5
md5:676f146e6b3d1408b0dcedf9386b21a8
776.0 MB Download
locust20010124a.hdf5
md5:ed7a300f9b525d547b84aa0ac13da37d
513.5 MB Download
locust20010124b_part1.hdf5
md5:4f352a95f254b8e0d7d34f5f3ee4e1f8
1.8 GB Download
locust20010124b_part2.hdf5
md5:f60bfc85df767ad7fb075ca9a0b81b1f
418.1 MB Download
locust20010131.hdf5
md5:621ba28036c016c9a886c2c2e6483176
600.4 MB Download
locust20010201.hdf5
md5:13f490b90099d73d305d362651b9a625
840.1 MB Download
locust20010208a.hdf5
md5:8154dd94457b23b19ce2565407798a59
937.4 MB Download
locust20010208b.hdf5
md5:1fc083fde64c2a3ffd2d4a85dd5910b0
1.4 GB Download
locust20010214_part1.hdf5
md5:30fcb09f0028c1b28acbf478ed1fba74
1.6 GB Download
locust20010214_part2.hdf5
md5:c48f2c99ab101e0462c1ca18c2ad5008
1.2 GB Download
locust20010217.hdf5
md5:2eb0f064b87ddfb0002719b02406c938
490.9 MB Download
  • Christophe Pouzat and Georgios Is Detorakis (2014) SPySort: Neuronal Spike Sorting with Python Proceedings of the 7th European Conference on Python in Science (EuroSciPy 2014), edited by Pierre de Buyl and Nelle Varoquaux, pp. 27–34 (arXiv:1412.6383).
  • Pouzat, Mazor and Laurent (2002) Journal of Neuroscience Methods 122(1): 43--57.

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