Published July 7, 2026
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Drechslerosporium cornellii gen. et sp. nov. within the Basidiobolaceae exhibiting unique conidial discharge and digitate chlamydospores
Authors/Creators
- 1. Anhui Provincial Key Laboratory for Microbial Pest Control, Anhui Agricultural University, Hefei, China|School of Civil Engineering and Architecture, Anhui University of Technology, Ma'anshan, China
- 2. Anhui Provincial Key Laboratory for Microbial Pest Control, Anhui Agricultural University, Hefei, China
Description
We describe Drechslerosporium cornellii gen. et sp. nov., a phylogenetically distinct fungal genus isolated from decaying plant litter at Cornell Plantations, Ithaca, New York, USA. This novel monotypic genus is assigned to the family Basidiobolaceae and is characterized by two unique morpho-developmental traits: (1) retention of prominent conidiophore appendages on discharged conidia following circumscissile rupture of the apical swelling and (2) direct ontogeny of digitate chlamydospores from primary conidia, a trait unrecorded in related genera. Multilocus phylogenetic analysis of nuclear ribosomal 28S, ITS, and RPB2 sequences robustly resolves Drechslerosporium as a sister lineage to Basidiobolus within Basidiobolaceae, yet forming a distinct evolutionary clade. This phylogenetic placement, coupled with its unique morphological characteristics, necessitates formal recognition of Drechslerosporium as an independent genus. Accordingly, a revised taxonomic circumscription of the Basidiobolaceae is provided to accommodate the newly discovered phenotypic and phylogenetic diversity. These findings clarify specialized discharge mechanisms driving spore dispersal evolution and expand our understanding of structural diversification within early-diverging lineages of fungi.
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- Al-Hatmi AMS, Balkhair A, Al-Busaidi I, Sandoval-Denis M, Al-Housni S, Ba Taher H, Al Shehhi AH, Raniga S, Al Shaibi M, Al Siyabi T, Meis JF, de Hoog GS, Al-Rawahi A, Al Muharrmi Z, Al-Harrasi A, Al Adawi B (2021) Basidiobolus omanensis sp. nov. Causing Angioinvasive Abdominal Basidiobolomycosis. Journal of Fungi 7: 653. https://doi.org/10.3390/jof7080653
- Ben-Ze'ev I, Kenneth RG (1982) Features-criteria of taxonomic value in the Entomophthorales: I. A revision of the Batkoan classification. Mycotaxon 14: 393–455. https://doi.org/10.5962/p.417381
- Carleton JP, Bradshaw AJ, Cleary LP, Hincher MR, Bushley K, Tabima JF (2026) Unraveling the genomic and phylogenetic complexity of the understudied microfungus Basidiobolus: Insights from 19 newly sequenced genomes. Mycologia 118(3): 482–495. https://doi.org/10.1080/00275514.2026.2619989
- Cavalier-Smith T (1987) The origin of fungi and pseudofungi. In: Rayner ADM, Brasier CM, Moore DM (Eds) Evolutionary biology of the fungi. Symposia of the British Mycological Society 13: 339–353. https://doi.org/10.1016/s0269-915x(87)80030-7
- Cavalier-Smith T (1998) A revised six-kingdom system of life. Biological Reviews 73: 203–266. https://doi.org/10.1111/j.1469-185x.1998.tb00030.x
- Davis WJ, Amses KR, Benny GL, Carter-House D, Chang Y, Grigoriev I, Smith ME, Spatafora JW, Stajich JE, James TY (2019) Genome-scale phylogenetics reveals a monophyletic Zoopagales (Zoopagomycota, Fungi). Molecular Phylogenetics and Evolution 133: 152–163. https://doi.org/10.1016/j.ympev.2019.01.006
- Drechsler C (1964) An odorous Basidiobolus often producing conidia plurally and forming some diclinous sexual apparatus. American Journal of Botany 51(7): 770–777. https://doi.org/10.1002/j.1537-2197.1964.tb06699.x
- Eidam E (1886) Basidiobolus eine neue gattung der Entomophthoracean. Beitrage zur Biologie der Pflanzen 4: 181–251.
- Galindo LJ, López-García P, Torruella G, Karpov S, Moreira D (2021) Phylogenomics of a new fungal phylum reveals multiple waves of reductive evolution across Holomycota. Nature Communications 12: 4973. https://doi.org/10.1038/s41467-021-25308-w
- Gryganskyi AP, Humber RA, Smith ME, Miadlikovska J, Wu S, Voigt K, Walther G, Anishchenko IM, Vilgalys R (2012) Molecular phylogeny of the Entomophthoromycota. Molecular Phylogenetics and Evolution 65: 682–694. https://doi.org/10.1016/j.ympev.2012.07.026
- Gugnani HC, Okafor JI (1980) Mycotic flora of the intestine and other internal organs of certain reptiles and amphibians with special reference to characterization of Basidiobolus isolates. Mycoses 23(5): 260–268. https://doi.org/10.1111/j.1439-0507.1980.tb02605.x
- Hibbett DS, Binder M, Bischoff JF, Blackwell M, Cannon PF, Eriksson OE, Huhndorf S, James T, Kirk PM, Lücking R, Thorsten Lumbsch H, Lutzoni F, Matheny PB, McLaughlin DJ, Powell MJ, Redhead S, Schoch CL, Spatafora JW, Stalpers JA, Vilgalys R, Aime MC, Aptroot A, Bauer R, Begerow D, Benny GL, Castlebury LA, Crous PW, Dai Y-C, Gams W, Geiser DM, Griffith GW, Gueidan C, Hawksworth DL, Hestmark G, Hosaka K, Humber RA, Hyde KD, Ironside JE, Kõljalg U, Kurtzman CP, Larsson K-H, Lichtwardt R, Longcore J, Miądlikowska J, Miller A, Moncalvo J-M, Mozley-Standridge S, Oberwinkler F, Parmasto E, Reeb V, Rogers JD, Roux C, Ryvarden L, Sampaio JP, Schüßler A, Sugiyama J, Thorn RG, Tibell L, Untereiner WA, Walker C, Wang Z, Weir A, Weiss M, White MM, Winka K, Yao Y-J, Zhang N (2007) A higher-level phylogenetic classification of the Fungi. Mycological Research 111: 509–547. https://doi.org/10.1016/j.mycres.2007.03.004
- Humber RA (1989) Synopsis of a revised classification for the Entomophthorales (Zygomycotina). Mycotaxon 36: 441–460. https://doi.org/10.5962/p.416715
- Humber RA (2012) Entomophthoromycota: a new phylum and reclassification for entomophthoroid fungi. Mycotaxon 120: 477–492. https://doi.org/10.5248/120.477
- James TY, Kauff F, Schoch CL, Matheny PB, Hofstetter V, Cox CJ, Celio G, Gueidan C, Fraker E, Miadlikowska J, Lumbsch HT, Rauhut A, Reeb V, Arnold AE, Amtoft A, Stajich JE, Hosaka K, Sung G-H, Johnson D, O'Rourke B, Crockett M, Binder M, Curtis JM, Slot JC, Wang Z, Wilson AW, Schüßler A, Longcore JE, O'Donnell K, Mozley-Standridge S, Porter D, Letcher PM, Powell MJ, Taylor JW, White MM, Griffith GW, Davies DR, Humber RA, Morton JB, Sugiyama J, Rossman AY, Rogers JD, Pfister DH, Hewitt D, Hansen K, Hambleton S, Shoemaker RA, Kohlmeyer J, Volkmann-Kohlmeyer B, Spotts RA, Serdani M, Crous PW, Hughes KW, Matsuura K, Langer E, Langer G, Untereiner WA, Lücking R, Büdel B, Geiser DM, Aptroot A, Diederich P, Schmitt I, Schultz M, Yahr R, Hibbett DS, Lutzoni F, McLaughlin DJ, Spatafora JW, Vilgalys R (2006) Reconstructing the early evolution of Fungi using a six-gene phylogeny. Nature 443: 818–823. https://doi.org/10.1038/nature05110
- Jensen AB, Gargas A, Eilenberg J, Rosendahl S (1998) Relationships of the insect-pathogenic order Entomophthorales (Zygomycota, Fungi) based on phylogenetic analyses of nucleus small subunit ribosomal DNA sequences (SSU rDNA). Fungal Genetics and Biology 24(3): 325–334. https://doi.org/10.1006/fgbi.1998.1063
- Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285
- Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
- Keeling PJ (2003) Congruent evidence from alpha-tubulin and beta-tubulin gene phylogenies for a zygomycete origin of microsporidia. Fungal Genetics and Biology 38: 298–309. https://doi.org/10.1016/s1087-1845(02)00537-6
- King DS (1976a) Systematics of Conidiobolus (Entomophthorales) using numerical taxonomy. I. Biology and cluster analysis. Canadian Journal of Botany 54: 45–65. https://doi.org/10.1139/b76-008
- King DS (1976b) Systematics of Conidiobolus (Entomophthorales) using numerical taxonomy. II. Taxonomic considerations. Canadian Journal of Botany 54: 1285–1296. https://doi.org/10.1139/b76-141
- Lutzoni F, Kauff F, Cox CJ, McLaughlin D, Celio G, Dentinger B, Padamsee M, Hibbett D, James TY, Baloch E, Grube M, Reeb V, Hofstetter V, Schoch C, Arnold AE, Miadlikowska J, Spatafora J, Johnson D, Hambleton S, Crockett M, Shoemaker R, Sung G, Lücking R, Lumbsch T, O'Donnell K, Binder M, Diederich P, Ertz D, Gueidan C, Hansen K, Harris RC, Hosaka K, Lim Y, Matheny B, Nishida H, Pfister D, Rogers J, Rossman A, Schmitt I, Sipman H, Stone J, Sugiyama J, Yahr R, Vilgalys R (2004) Assembling the fungal tree of life: Progress, classification, and evolution of subcellular traits. American Journal of Botany 91: 1446–1480. https://doi.org/10.3732/ajb.91.10.1446
- Nagahama T, Sato H, Shimazu M, Sugiyama J (1995) Phylogenetic divergence of the entomophthoralean fungi: evidence from nuclear 18S ribosomal RNA gene sequences. Mycologia 87: 203–209. https://doi.org/10.1080/00275514.1995.12026522
- Nguyen L-T, Schmidt HA, von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32(1): 268–274. https://doi.org/10.1093/molbev/msu300
- Nie Y, Zhao H, Zhang M, Lee H, Chang Y, Liu X, Huang B (2025) Updating taxonomy of Conidiobolus sensu lato: Proposal of a new family, a new genus, 23 new species and two new combinations. Mycosphere 16(1): 4594–4662. https://doi.org/10.5943/mycosphere/16/1/34
- Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817–818. https://doi.org/10.1093/bioinformatics/14.9.817
- Rambaut A (2012) FigTree version 1.4.0. http://tree.bio.ed.ac.uk/software/figtree/
- Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
- Sitterlé E, Rodriguez C, Mounier R, Calderaro J, Foulet F, Develoux M, Pawlotsky J-M, Botterel F (2017) Contribution of ultra deep sequencing in the clinical diagnosis of a new fungal pathogen species: Basidiobolus meristosporus. Frontiers in Microbiology 8: 334. https://doi.org/10.3389/fmicb.2017.00334
- Spatafora JW, Chang Y, Benny GL, Lazarus K, Smith ME, Berbee ML, Bonito G, Corradi N, Grigoriev I, Gryganskyi A, James TY, O'Donnell K, Roberson RW, Taylor TN, Uehling J, Vilgalys R, White MM, Stajich JE (2016) A phylum-level phylogenetic classification of zygomycete fungi based on genome-scale data. Mycologia 108: 1028–1046. https://doi.org/10.3852/16-042
- Spatafora JW, Aime MC, Grigoriev IV, Martin F, Stajich JE, Blackwell M (2017) The fungal tree of life: from molecular systematics to genome-scale phylogenies. Microbiology Spectrum 5(5): FUNK-0053-2016. https://doi.org/10.1128/microbiolspec.funk-0053-2016
- Strassert JFH, Monaghan MT (2022) Phylogenomic insights into the early diversification of fungi. Current Biology 32: 3628–3635.e3. https://doi.org/10.1016/j.cub.2022.06.057
- Tanabe Y, Saikawa M, Watanabe MM, Sugiyama J (2003) Molecular phylogeny of Zygomycota based on EF-1a and RPB1 sequences: limitations and utility of alternative markers to rDNA. Molecular Phylogenetics and Evolution 30: 438–449. https://doi.org/10.1016/s1055-7903(03)00185-4
- Tanabe Y, O'Donnell K, Saikawa M, Sugiyama J (2000) Molecular phylogeny of parasitic Zygomycota (Dimargaritales, Zoopagales) based on nuclear small subunit ribosomal DNA sequences. Molecular Phylogenetics and Evolution 16: 253–262. https://doi.org/10.1006/mpev.2000.0775
- Vaidya G, Lohman DJ, Meier R (2011) SequenceMatrix: Concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics 27(2): 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
- Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
- Wang Y, Nie Y, Yu D, Xie X, Qin L, Yang Y, Huang B (2020) Genome-wide study of saprotrophy-related genes in the basal fungus Conidiobolus heterosporus. Applied Microbiology and Biotechnology 104(14): 6261–6272. https://doi.org/10.1007/s00253-020-10698-6
- Wang Y, Chang Y, Ortañez J, Peña JF, Carter-House D, Reynolds NK, Smith ME, Benny G, Mondo SJ, Salamov A, Lipzen A, Pangilinan J, Guo J, LaButti K, Andreopolous W, Tritt A, Keymanesh K, Yan M, Barry K, Grigoriev IV, Spatafora JW, Stajich JE (2023) Divergent evolution of early terrestrial fungi reveals the evolution of mucormycosis pathogenicity factors. Genome Biology and Evolution 15: evad046. https://doi.org/10.1093/gbe/evad046
- White T, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds) PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
- Zhu H, Qu F, Zhu LH (1994) Isolation of genomic DNAs from fungi using benzyl chloride. Acta Mycologica Sinica 13: 41–47. https://doi.org/10.1093/nar/21.22.5279