Published May 26, 2026
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Two new Inosperma (Inocybaceae) species from the karst forest of Guangxi, China
Authors/Creators
- 1. Nonggang Karst Ecosystem Observation and Research Station of Guangxi, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, China
- 2. Engineering Research Center of Tropical Medicine Innovation and Transformation of Ministry of Education, Hainan Provincial Key Laboratory of Research and Development on Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou, China
- 3. Administrative Centre of Guangxi Mulun National Nature Reserve, Huanjiang, China
Description
In this study, we described two new species, Inosperma nonggangense Y.G. Fan, G.F. Mou & S. Li, sp. nov. and Inosperma rufobrunneum Y.G. Fan, G.F. Mou & S. Li, sp. nov. from Guangxi, China. These species were identified based on morphological and multi-locus (ITS, LSU, and rpb2) phylogenetic analyses. Inosperma nonggangense sp. nov. is characterized by yellowish to orange basidiomata, rimulose pileus, crowded lamellae, ovoid to ellipsoid basidiospores measuring 8.7–9.9 × 5.2–6.1 μm, thin-walled, broadly clavate cheilocystidia, and abundant oily hyphae in hymenophoral, pileal, and stipe trama. Inosperma rufobrunneum sp. nov. is characterized by reddish-brown basidiomata at maturity, rimulose to rimose pileus, crowded lamellae, nearly smooth stipes, ellipsoid basidiospores measuring 8.2–9.3 × 4.9–5.4 μm, and thin-walled, clavate cheilocystidia. Phylogenetically, these species belong to the Old World tropical clade 2, formed distinct independent lineages.
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References
- Aïgnon HL, Jabeen S, Naseer A, Yorou NS, Ryberg M (2021) Three new species of Inosperma (Agaricales, Inocybaceae) from tropical Africa. MycoKeys 77: 97–116. https://doi.org/10.3897/mycokeys.77.60084
- Aïgnon HL, Fan YG, De Kesel A, Bahram M, Ryberg M, Yorou NS (2023) A new species of Inosperma, and first record of I. afromelliolens (Inocybaceae, Fungi) from West Africa. PLOS ONE 18(10): e0290894. https://doi.org/10.1371/journal.pone.0290894
- Bandini D, Oertel B, Eberhardt U (2021) Even more fibre-caps (2): Thirteen new species of the family Inocybaceae. Mycologia Bavarica 21: 27–98.
- Bandini D, Oertel B, Eberhardt U (2022) Even more fibre-caps (3): Twenty-one new species of the family Inocybaceae. Mycologia Bavarica 22: 31–138. https://doi.org/10.18476/2022.901982
- Bao DF, Tian XG, Samarakoon MC, Karunarathna SC, Luo ZL, Han JJ, Wen TC, He ZJ, Liu ZH, Lu YZ, Kang JC (2025) Biodiversity of lignicolous freshwater fungi from the Nanpan river basin in Guizhou and Guangxi provinces, China, with descriptions of fifteen species. Mycosphere 16(1): 3695–3752. https://doi.org/10.5943/mycosphere/16/1/29
- Bau T, Fan YG (2018) Three new species of Inocybe sect. Rimosae from China. Mycosystema 37(6): 693–702.
- Bizio E, Castellan A (2017) Inocybe acutofulva e Inocybe grammopodia var. paleoveneta, duenuovi taxa dall'alta marca trevigiana (Treviso, Veneto, Italia). Micologia Vegetazione Mediterranea 32(2): 103–124.
- Cervini M, Carbone M, Bizio E (2020) Inosperma vinaceum, una nuova specie distinta da I. rhodiolum e I. adaequatum. Rivista di Micologia 63(3): 215–241.
- Chandrasekharan B, Pradeep C, Vrinda B (2020) Inocybe poisoning from Kerala—a case study. Journal of Mycological Research 57(4): 255–258.
- Chen HL, Huang YS (2025) General report on new taxa of vascular plants in Guangxi. Guihaia 45(3): 396–405. https://doi.org/10.11931/guihaia.gxzw202502033
- Crous P, Jurjevi Z, Balashov S, Pinruan AM, Rigueiro-Rodriguez A, Osieck ER, Altes A, Czachura P, Esteve-Raventos F, Gunaseelan S, Kaliyaperumal M, Larsson E, Luangsa-Ard JJ, Moreno G, Pancorbo F, Pitek M, Sommai S, Somrithipol S, Asif M, Delgado G, Flakus A, Illescas T, Kezo K, Khamsuntorn P, Kubatova A, Labuda R, Lavoise C, Lebel T, Lueangjaroenkit P, Macia-Vicente J, Paz A, Saba M, Shivas R, Tan Y, Wingfield M, Aas T, Abramczyk B, Ainsworth A, Akulov A, Alvarado P, Armada F, Assyov B, Avchar R, Avesani M, Bezerra J, Bhat J, Bilaski P, Bily D, Boccardo F, Bozok F, Campos J, Chaimongkol S, Chellappan N, Costa M, Dalecka M, Darmostuk V, Daskalopoulos V, Dearnaley J, Dentinger B, Silva ND, Dhotre D, Carlavilla J, Doungsa-ard C, Dovana F, Erhard A, Ferro L, Gallegos S, Giles C, Gore G, Gorfer M, Guard F, Hanson S, Haridev P, Jankowiak R, Jeffers S, Kandemir H, Karich A, Kiso K, Kiss L, Krisai-Greilhuber I, Latha K, Lorenzini M, Lumyong S, Manimohan P, Manjon J, Maula F, Mazur E, Mesquita N, Mynek K, Mongkolsamrit S, Moran P, Murugadoss R, Nagarajan M, Nalumpang S, Noisripoom W, Nosalj S, Novaes Q, Nowak M, Pawowska J, Peiger M, Pereira O, Pinto A, Plaza M, Polemis E, Polhorsky A, Ramos D, Raza M, Rivas-Ferreiro M, Rodriguez-Flakus P, Ruszkiewicz-Michalska M, Sanchez A, Santos A, Schuller A, Scott P, Shelke D, Liwa L, Solheim H, Sonawane H, Straiftakova D, Stryjak-Bogacka M, Sudsanguan M, Suwannarach N, Suz L, Syme K, Takn H, Tennakoon D, Tomka P, Vaghefi N, Vasan V, Vauras J, Wiktorowicz D, Villarreal M, Vizzini A, Wrzosek M, Yang X, Yingkunchao W, Zapparoli G, Zervakis G, Groenewald J (2024) Fungal Planet description sheets: 1614–1696. Fungal Systematics and Evolution 13: 183–440. https://doi.org/10.3114/fuse.2024.13.11
- Deng LS, Yu WJ, Zeng NK, Liu LJ, Liu LY, Fan YG (2021a) Inosperma subsphaerosporum (Inocybaceae), a new species from Hainan, tropical China. Phytotaxa 502: 169–178. https://doi.org/10.11646/phytotaxa.502.2.5
- Deng LS, Kang R, Zeng NK, Yu WJ, Chang C, Xu F, Deng WQ, Qi LL, Zhou YL, Fan YG (2021b) Two new Inosperma (Inocybaceae) species with unexpected muscarine contents from tropical China. MycoKeys 85: 87–108. https://doi.org/10.3897/mycokeys.85.71957
- Deng LS, Yu WJ, Zeng NK, Zhang YZ, Wu XP, Li HJ, Xu F, Fan YG (2022) A new muscarine-containing Inosperma (Inocybaceae, Agaricales) species discovered from one poisoning incident occurring in tropical China. Frontiers in Microbiology 13: 923435. https://doi.org/10.3389/fmicb.2022.923435
- Esteve-Raventós F, Larsson E, Pancorbo F, Bizio E, Altés A, Turégano Y, Moreno G, Olariaga I (2024) A taxonomic and phylogenetic contribution on Inosperma section Inosperma (Agaricales, Inocybaceae) in Europe: Calamistratum and Geraniodorum Groups. Journal of Fungi 10(6): 374. https://doi.org/10.3390/jof10060374
- Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes– application to the identification of mycorrhizae and rusts. Molecular Ecology 2(2): 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
- Herrmann M (1964) Die Naumburger Massen-Pilzvergiftung mit dem Ziegelroten Rißpilz–Inocybe patouillardii. Mykologisches Mitteilungsblatt 8: 42–44.
- Horak E, Matheny PB, Desjardin DE, Soytong K (2015) The genus Inocybe (Inocybaceae, Agaricales, Basidiomycota) in Thailand and Malaysia. Phytotaxa 230(3): 201–238. https://doi.org/10.11646/phytotaxa.230.3.1
- Hall TA (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41(41): 95–98.
- Kuyper TW (1986) A revision of the genus Inocybe in Europe. I. Subgenus Inosperma and the smooth-spored species of subgenus Inocybe. Persoonia Supplement 3: 1–247.
- Kornerup A, Wanscher JH, Pavey D (1978) Methuen handbook of colour. Methuen Publishing Ltd, London, 256 pp.
- Kühner R (1980) Les Hymenomycetes agaricoides. Bulletin Mensuel de la Societe Linneenne de Lyon 49: 11027.
- Kropp BR, Matheny PB, Hutchison LJ (2013) Inocybe section Rimosae in Utah: phylogenetic affinities and new species. Mycologia 105(3): 728–747. https://doi.org/10.3852/12-185
- Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33(7): 1870–1874. https://doi.org/10.1093/molbev/msw054
- Lurie Y, Wasser SP, Taha M, Shehade H, Nijim J, Hoffmann Y, Basis F, Moshe V, Lavon O, Suaed S, Bishara B, Bentur Y (2009) Mushroom poisoning from species of genus Inocybe (fiber head mushroom): A case series with exact species identification. Clinical Toxicology 47: 562–565. https://doi.org/10.1080/15563650903008448
- Latha KPD, Manimohan P (2016) Inocybe gregaria, a new species of the Inosperma clade from tropical India. Phytotaxa 286(2): 107–115. https://doi.org/10.11646/phytotaxa.286.2.5
- Latha KPD, Manimohan P (2017) Inocybes of Kerala. SporePrint Books, Calicut-India, 181 pp.
- Li HJ, Zhang HS, Zhang YZ, Zhou J, Yin Y, He Q, Jiang SF, Ma PB, Zhang YT, Wen K, Yuan Y, Lang N, Cheng BW, Lu JJ, Sun CY (2021) Mushroom poisoning outbreaks-China, 2020. China CDC Weekly 3(3): 41–45. https://doi.org/10.46234/ccdcw2021.014
- Li S, Xu F, Long P, Zhang P, Fan YG, Chen ZH (2022) Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection. Microbiology 13: 1021583. https://doi.org/10.3389/fmicb.2022.1021583
- Liu LL, Ren YL, Habib K, Lu CT, Wu YP, Long SH, Lin Y, Zhang X, Kang YQ, Wijayawardene NN, Wang F, Elgorban AM, Rajaei AI, Samarakoon MC, Shen XC, Li QR (2025) New taxa of Xylariales from Karst Ecosystems in Southwestern China. Mycosphere 16(1): 1–78. https://doi.org/10.5943/mycosphere/16/1/1
- Matheny PB (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe, Agaricales). Molecular Phylogenetics and Evolution 35(1): 1–20. https://doi.org/10.1016/j.ympev.2004.11.014
- Matheny PB, Curtis JM, Hofstetter V, Aime MC, Moncalvo JM, Ge ZW, Slot JC, Ammirati JF, Baroni TJ, Bougher NL, Hughes KW, Lodge DJ, Kerrigan RW, Seidl MT, Aanen DK, DeNitis M, Daniele GM, Desjardin DE, Kropp BR, Norvell LL, Hibbett DS (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98(6): 982–995. https://doi.org/10.1080/15572536.2006.11832627
- Matheny PB, Moreau P-A (2009a) A rare and unusual lignicolous species of Inocybe (Agaricales) from eastern North America. Brittonia 61: 163–171. https://doi.org/10.1007/s12228-008-9066-4
- Matheny PB, Aime MC, Bougher NL, Buyck B, Desjardin DE, Horak E, Kropp BR, Lodge DJ, Soytong K, Trappe JM, Hibbett DS (2009b) Out of the Palaeotropics? Historical biogeography and diversification of the cosmopolitan ectomycorrhizal mushroom family Inocybaceae. Journal of Biogeography 36(4): 577–592. http://www.jstor.org/stable/20488391
- Marchetti M, Franchi P, Consiglio G (2014) Typification of some of Britzelmayr's Inocybe species. Revista Iberoamericana De Micología 2014(2): 127–178.
- Matheny PB, Henkel TW, Séné O, Korotkin HB, Dentinger BTM, Aime MC (2017) New species of Auritella (Inocybaceae) from Cameroon, with a worldwide key to the known species. IMA Fungus 8: 287–298. https://doi.org/10.5598/imafungus.2017.08.02.06
- Matheny PB, Hobbs AM, Esteve-Raventos F (2020) Genera of Inocybaceae: New skin for the old ceremony. Mycologia 112(1): 83–120. https://doi.org/10.1080/00275514.2019.1668906
- Naseer A (2018) Inocybe shawarensis sp. nov. in the Inosperma clade from Pakistan. Mycotaxon 132(4): 909–918. https://doi.org/10.5248/132.909
- Naseer A, Khurshid R, Fan YG, Khalid AN (2025) Morphology and multigene phylogeny reveal a new species of Inosperma from Himalayas of Pakistan. BMC Microbiology 25: 688. https://doi.org/10.1186/s12866-025-03972-y
- Nylander J (2004) MrModeltest V2. program distributed by the author. Bioinformatics 24: 581–583.
- Pradeep CK, Vrinda KB, Varghese SP, Korotkin HB, Matheny PB (2016) New and noteworthy species of Inocybe (Agaricales) from tropical India. Mycological Progress 15: 24. https://doi.org/10.1007/s11557-016-1174-z
- Parnmen S, Nooron N, Leudang S, Sikaphan S, Polputpisatkul D, Pringsulaka O, Binchai S, Rangsiruji A (2021) Foodborne illness caused by muscarine-containing mushrooms and identification of mushroom remnants using phylogenetics and LC–MS/MS. Food Control 128(4): 108182. https://doi.org/10.1016/j.foodcont.2021.108182
- Rathnayaka AR, Tennakoon DS, Jones GE, Wanasinghe DN, Bhat DJ, Priyashantha AH, Stephenson SL, Tibpromma S, Karunarathna SC (2025) Significance of precise documentation of hosts and geospatial data of fungal collections, with an emphasis on plant-associated fungi. New Zealand Journal of Botany 63(2–3): 462–489. https://doi.org/10.1080/0028825X.2024.2381734
- Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61(3): 539–542. https://doi.org/10.1093/sysbio/sys029
- Sana S, Kiran M, Ali W (2025) Inosperma pakistanicum sp. nov. (Inocybaceae, Agaricales) from Swat, Pakistan. Phytotaxa 690(1): 77–88. https://doi.org/10.11646/phytotaxa.690.1.6
- Tan WN, Luo LJ, Nong SY, Hang XQ, Liu Y, Huang YS (2023) Primary study on species diversity of plant in Guangxi Mulun national nature reserve. Guihaia 43(12): 2182–2195.
- Trifinopoulos J, Nguyen LT, von Haeseler A, Minh BQ (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research 44(W1): 1–4. https://doi.org/10.1093/nar/gkw256
- Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172(8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
- Wu XL, Tan WF, Song B, Peng DR, Wu WH (2021) Guangxi macrofungus in China. China Forestry Publishing House, Beijing, 447 pp.
- White J, Weinstein S, Haro LD, Bédry R, Schaper A, Rumack BH, Zilker T (2018) Mushroom poisoning: A proposed new clinical classification. Toxicon 157: 53–65. https://doi.org/10.1016/j.toxicon.2018.11.007
- Zhou YL, Deng LS, Yang SD, Liu CF, Fan YG, Yu WJ (2023) Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China. Microbiology 14: 1326253. https://doi.org/10.3389/fmicb.2023.1326253