Published November 14, 2025 | Version v1
Journal article Open

Two new species of Chionosphaera and Kurtzmanomyces (Chionosphaeraceae, Agaricostilbomycetes) isolated from China

  • 1. Nanyang Normal University, Nanyang, China

Description

Within the family Chionosphaeraceae, only nine species of Chionosphaera and Kurtzmanomyces have been described to date. In this study, two strains of Chionosphaera and two strains of Kurtzmanomyces from China were examined using phylogenetic, physiological, and biochemical approaches. Phylogenetic analyses based on sequences of the internal transcribed spacer (ITS) region, the D1/D2 domain of the large subunit (LSU) rRNA gene, and the translation elongation factor 1-α gene (tef1α) were conducted to infer species relationships within the genera Chionosphaera and Kurtzmanomyces. As a result, two novel species are proposed: Chionosphaera foliicola sp. nov. (holotype GDMCC 2.531T) and Kurtzmanomyces guiyangensis sp. nov. (holotype GDMCC 2.499T). Descriptions and illustrations of both species are provided, together with comparisons to closely related taxa. This study expands the known diversity of Chionosphaeraceae in China and provides a basis for future taxonomic and ecological investigations.

Files

MC_article_167837.pdf

Files (1.3 MB)

Name Size Download all
md5:8bb6be86995af65d9417bfc188be046d
1.1 MB Preview Download
md5:1e8d2c76f1ac08d53422feb953361b4c
166.1 kB Preview Download

Linked records

Additional details

References

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research 25(17): 3389–3402. https://doi.org/10.1093/nar/25.17.3389
  • Andrew R (2016) FigTree: Tree figure drawing tool Version 1.4.3. Institute of Evolutionary Biology, United Kingdom, University of Edinburgh Press.
  • Bandoni RJ (1998) On some species of Mycogloea. Mycoscience 39: 31–36. https://doi.org/10.1007/BF02461575
  • Bauer R, Begerow D, Sampaio JP, Weiß M, Oberwinkler F (2006) The simple-septate basidiomycetes: A synopsis. Mycological Progress 5: 41–66. https://doi.org/10.1007/s11557-006-0502-0
  • Cox DE (1976) A new homobasidiomycete with anomalous basidia. Mycologia 68: 481–510. https://doi.org/10.1080/00275514.1976.12019937
  • do Carmo-Sousa L, Phaff HJ (1962) An improved method for the detection of spore discharge in the Sporobolomycetaceae. Journal of Bacteriology 83(2): 434–435. https://doi.org/10.1128/jb.83.2.434-435.1962
  • Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A (2000) Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. International Journal of Systematic and Evolutionary Microbiology 50(3): 1351–1371. https://doi.org/10.1099/00207713-50-3-1351
  • Giménez-Jurado G, Plácido T, Cidadão AJ, Cabeça-Silva C, Fonseca E, Roeijmans HJ, van Eijk GW, van Uden N (1990) Kurtzmanomyces tardus sp. nov., a new anamorphic yeast species of basidiomycetous affinity. Antonie van Leeuwenhoek 58: 129–135. https://doi.org/10.1007/BF00422729
  • Hall TA (1999) Bioedit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  • Hibbett DS (2006) A phylogenetic overview of the Agaricomycotina. Mycologia 98(6): 917–925. https://doi.org/10.3852/mycologia.98.6.917
  • Jiang YL, Bao WJ, Liu F, Wang GS, Yurkov AM, Ma Q, Hu ZD, Chen XH, Zhao WN, Li AH, Wang QM (2024) Proposal of one new family, seven new genera and seventy new basidiomycetous yeast species mostly isolated from Tibet and Yunnan provinces, China. Studies in Mycology 109: 57–153. https://doi.org/10.3114/sim.2024.109.02
  • Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution 30(4): 772–780. https://doi.org/10.1093/molbev/mst010
  • Kirschner R, Chen CJ (2008) Physalacria corticola, P. stilboidea, and Stilbum vulgare, three Sphaeropedunculate basidiomycota new to taiwan, with notes on Stilbum erythrinae. Fungal Science 23: 43–53.
  • Kirschner R, Begerow D, Oberwinkler F (2001) A new Chionosphaera species associated with conifer inhabiting bark beetles. Mycological Research 105: 1403–1408. https://doi.org/10.1017/S0953756201005019
  • Kirschner R, Sampaio JP, Begerow D, Chen ZC, Oberwinkler F (2003) Mycogloea nipponica–the first known teleomorph in the heterobasidiomycetous yeast genus Kurtzmanomyces. Antonie van Leeuwenhoek 84(2): 109–114. https://doi.org/10.1023/a:1025484800974
  • Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4): 331–371. https://doi.org/10.1023/a:1001761008817
  • Kurtzman CP, Fell JW, Boekhout T, Robert V (2011) Methods for isolation, phenotypic characterization and maintenance of yeasts. In: Kurtzman CP, Fell JW, Boekhout T (Eds) The Yeasts – a Taxonomic Study, 5th edn, vol. 1. Elsevier, Amsterdam, 87–110. https://doi.org/10.1016/B978-0-444-52149-1.00007-0
  • Kyung J, Kwon-Chung (2011) Chionosphaera Cox (1976). In: Kurtzman CP, Fell JW, Boekhout T (Eds) The Yeasts – a Taxonomic Study, 5th edn, vol. 1. Amsterdam, Elsevier, 1395–1401. https://doi.org/10.1016/B978-0-444-52149-1.00106-3
  • Lee S, Ahn C, Kim YJ, Choi SM, Ko JW, Kim C, Park CS (2025) Chionosphaera pinicorticola sp. nov., a novel basidiomycetous yeast species isolated from pine tree bark in Gyeongju, South Korea. International Journal of Systematic and Evolutionary Microbiology 75(1): 00662201. https://doi.org/10.1099/ijsem.0.006622
  • Li AH, Yuan FX, Groenewald M, Bensch K, Yurkov AM, Li K, Han PJ, Guo LD, Aime MC, Sampaio JP, Jindamorakot S, Turchetti B, Inacio J, Fungsin B, Wang QM, Bai FY (2020) Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species. Studies in Mycology 96: 17–140. https://doi.org/10.1016/j.simyco.2020.01.002
  • Millanes AM, Diederich P, Westberg M, Wedin M (2021) Crittendenia gen. nov., a new lichenicolous lineage in the Agaricostilbomycetes (Pucciniomycotina), and a review of the biology, phylogeny and classification of lichenicolous heterobasidiomycetes. Lond 53: 103–116. https://doi.org/10.1017/S002428292000033X
  • Oberwinkler F, Bandoni RJ (1982) A taxonomic survey of the gasteroid, auricularioid heterobasidiomycetes. Canadian Journal of Botany 60: 1726–1750. https://doi.org/10.1139/b82-221
  • Posada D (2008) jModelTest: Phylogenetic model averaging. Molecular Biology and Evolution 25(7): 1253–1256. https://doi.org/10.1093/molbev/msn083
  • Rehner SA, Buckley EA (2005) Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: Evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97(1): 84–98. https://doi.org/10.3852/mycologia.97.1.84
  • Rodrigues de Miranda L (1975) Two new species of the genus Sterigmatomyces. Antonie van Leeuwenhoek 41: 193–198. https://doi.org/10.1007/BF02565051
  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice, across a large model space. Systematic Biology 61(3): 539–542. https://doi.org/10.1093/sysbio/sys029
  • Sampaio JP (2011) Kurtzmanomyces Y. Yamada, M. ltoh, Kawasaki, Banno & Nakase (1988). In: Kurtzman CP, Fell JW, Boekhout T (Eds) The Yeasts – a Taxonomic Study, 5th ed. , vol. 3. Amsterdam, Elsevier, 1795–1800. https://doi.org/10.1016/B978-0-444-52149-1.00146-4
  • Sampaio JP, Fell JW, Gadanho M, Bauer R (1999) Kurtzmanomyces insolitus sp. nov., a new anamorphic heterobasidiomycetous yeast species. Systematic and Applied Microbiology 22: 619–625. https://doi.org/10.1016/S0723-2020(99)80015-0
  • Stamatakis A (2014) RAxML Version 8: A tool for phylogenetic analyses and post analyses of large phylogenies. Bioinformatics (Oxford, England) 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  • Toome M, Roberson RW, Aime MC (2013) Meredithblackwellia eburnea gen. et sp. nov., Kriegeriaceae fam. nov. and Kriegeriales ord. nov.—toward resolving higher-level classification in Microbotryomycetes. Mycologia 105: 486–495. https://doi.org/10.3852/12-251
  • Wang QM, Theelen B, Groenewald M, Bai FY, Boekhout T (2014) Moniliellomycetes and Malasseziomycetes, two new classes in Ustilaginomycotina. Persoonia 33: 41–47. https://doi.org/10.3767/003158514X682313
  • Wang QM, Yurkov AM, Göker M, Lumbsch HT, Leavitt SD, Groenewald M, Heelen B, Liu XZ, Boekhout T, Bai FY (2015) Phylogenetic classification of yeasts and related taxa within Pucciniomycotina. Studies in Mycology 81: 149–189. https://doi.org/10.1016/j.simyco.2015.12.002
  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds) PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, 315–322. http://doi.org/10.1016/B978-0-12-372180-8.50042-1
  • Yamada Y, Itoh M, Kawasaki H, Banno I, Nakase T (1988) Kurtzmanomyces gen. nov. an anamorphic yeast genus for the Q10-equipped organism whose conidium is freed by an end-break in the sterigma which branches or elongates to produce additional conidia and whose cells contain no xylose. The Journal of General and Applied Microbiology 34: 503–506. https://doi.org/10.2323/jgam.34.503
  • Zhang T, Jia RL, Zhang YQ, Liu HY, Su J, Yu LY (2013) Kurtzmanomyces shapotouensis sp. nov., an anamorphic, basidiomycetous yeast isolated from desert soil crusts. International Journal of Systematic and Evolutionary Microbiology 63(10): 3892–3895. https://doi.org/10.1099/ijs.0.053058-0
  • Zhang D, Gao F, Jakovlić I, Zou H, Zhang J, Li WX, Wang GT (2020) Phylosuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20(1): 348–355. https://doi.org/10.1111/1755-0998.13096