In vitro and in vivo effects of Amanita muscaria tincture on the a. basilaris contractility and on a rotenone-induced model of Parkinson's disease
Authors/Creators
- 1. Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria
- 2. Faculty of Veterinary Medicine, University of Forestry, Sofia, Bulgaria
- 3. Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria
- 4. Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
Description
Amanita muscaria is a well-known mushroom containing the GABAA receptor agonist muscimol, a compound of growing interest due to its potential neuroprotective properties. The present study investigated the in vivo neuroprotective effects of an A. muscaria tincture (AM) in a rotenone-induced mouse model of Parkinson's disease and evaluated its in vitro effects on the contractility of rat arteria basilaris. Muscimol content in the tincture was quantified by HPLC. Mice were treated with AM alone or in combination with rotenone for 30 days, after which oxidative stress markers and brain histology were assessed. Rotenone significantly increased malondialdehyde levels and lowered reduced glutathione concentrations in brain homogenates, accompanied by marked degenerative-necrotic changes. Co-treatment with AM significantly attenuated lipid peroxidation, preserved glutathione levels, and maintained brain histoarchitectonics. In vitro vascular studies demonstrated that both AM and pure muscimol (10 µM) significantly reduced the basal tone of isolated rat a. basilaris. These findings demonstrate a pronounced neuroprotective effect of A. muscaria tincture in a rotenone-induced model of Parkinsonism and reveal a vasorelaxant action of muscimol on cerebral arteries.
Files
PHAR_article_187461.pdf
Files
(1.2 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:c648e80435611e17306c108aaaa434e8
|
1.1 MB | Preview Download |
|
md5:774afb0487e8946f6fac1b91741927f9
|
48.6 kB | Preview Download |
Additional details
References
- Bai X, Qiu A, Guan J, Shi Z (2007) Antioxidant and protective effect of an oleanolic acid-enriched extract of A. deliciosa root on carbon tetrachloride induced rat liver injury. Asia Pacific journal of clinical nutrition 16 [Suppl 1]: 169–173.
- Czuczwar SJ, Chmielewska B, Turski WA, Kleinrok Z (1984) Differential effects of baclofen, gamma-hydroxybutyric acid and muscimol on the protective action of phenobarbital and diphenylhydantoin against maximal electroshock-induced seizures in mice. Neuropharmacology 23: 159–163. https://doi.org/10.1016/s0028-3908(84)80008-8
- Dauer W, Przedborski S (2003) Parkinson's disease: mechanisms and models. Neuron 39: 889–909. https://doi.org/10.1016/s0896-6273(03)00568-3
- Hessellund A, Jeppesen P, Aalkjaer C, Bek T (2003) Characterization of vasomotion in porcine retinal arterioles. Acta ophthalmologica Scandinavica 81: 278–282. https://doi.org/10.1034/j.1600-0420.2003.00063.x
- Kondeva-Burdina M, Voynova M, Shkondrov A, Aluani D, Tzankova V, Krasteva I (2019) Effects of Amanita muscaria extract on different in vitro neurotoxicity models at sub-cellular and cellular levels. Food and Chemical Toxicology 132: 110687. https://doi.org/10.1016/j.fct.2019.110687
- Koutsoviti-Papadopoulou M, Nikolaidis E, Kounenis G (2003) Enhancing and inhibitory effects of H2-receptor antagonists on the GABA and the GABAA-agonist muscimol responses of the isolated guinea pig ileum: a pharmacodynamic interaction. Pharmacological Research 48(3): 279–284. https://doi.org/10.1016/S1043-6618(03)00148-8
- Krogsgaard-Larsen P (1979) GABA-neurotransmitters pharmacochemical, biochemical and pharmacological aspects GABA-neurotransmitters: pharmacochemical, biochemical and pharmacological aspects. Munksgaard, Copenhagen.
- Mulvany MJ, Halpern W (1977) Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circulation research 41: 19–26. https://doi.org/10.1161/01.res.41.1.19
- Salama M, Sobh M, Emam M, Abdalla A, Sabry D, El-Gamal M, Lotfy A, El-Husseiny M, Sobh M, Shalash A, Mohamed WM (2017) Effect of intranasal stem cell administration on the nigrostriatal system in a mouse model of Parkinson's disease. Experimental and therapeutic medicine 13: 976–982. https://doi.org/10.3892/etm.2017.4073
- Scotti de Carolis A, Lipparini F, Longo VG (1969) Neuropharmacological investigations on muscimol, a psychotropic drug extracted from Amanita muscaria. Psychopharmacologia 15: 186–195. https://doi.org/10.1007/BF00411168
- Tamaoki J, Graf PD, Nadel JA (1987) Effect of gamma-aminobutyric acid on neurally mediated contraction of guinea pig trachealis smooth muscle. The Journal of pharmacology and experimental therapeutics 243: 86–90. https://doi.org/10.1016/s0022-3565(25)39257-8
- Vazharov V (2016) Medicinal fungi in Bulgaria. Biana, Sofia.
- Voynova M, Shkondrov A, Krasteva I, Kondeva-Burdina M (2021) In vitro effects of synthetic muscimol and an extract from Amanita muscaria on human recombinant MAOB enzyme. Pharmacia 68: 147–150. https://doi.org/10.3897/pharmacia.68.e60705.figure1