Published March 13, 2026 | Version v1
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Figure 2 from: Ivanov H, Linev A, Pochileev I, Stoyanova V (2026) Droplet digital PCR outperforms qPCR by revealing clinically relevant low-frequency KRAS mutations in colorectal cancer. Pharmacia 73: 1-7. https://doi.org/10.3897/pharmacia.73.e187140

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  • 1. Department of Medical Genetics, Medical University of Plovdiv, Plovdiv, Bulgaria

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Figure 2 ROC curve evaluating the ability of ddPCR-derived VAF values to predict qPCR mutation detection. AUC = 1.00 indicates perfect discrimination. The optimal VAF threshold distinguishing qPCR-positive from qPCR-negative samples was ~18%. The ROC analysis confirms that qPCR fails to detect mutations below its effective sensitivity range, whereas ddPCR reliably detects low-frequency variants down to ~1% VAF.

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Journal article: 10.3897/pharmacia.73.e187140 (DOI)
Journal article: https://zenodo.org/record/19029281 (URL)