Published November 30, 2018 | Version 1

Maintenance of spatial gene expression by Polycomb-mediated repression after formation of a vertebrate body plan

  • 1. Radboud University, Faculty of Science, Department of Molecular Biology, Radboud Institute for Molecular Life Sciences, Nijmegen, the Netherlands
  • 2. Howard Hughes Medical Institute, Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
  • 3. Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands

Description

This dataset contains zebrafish (Danio rerio) raw RNA and ChIP sequencing data:

  • RNA-seq:
    • RNAseq_Wildtype_rep[12].fastq.gz: 2 biological replicates of single-end RNA-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • RNAseq_Wildtype_rep[3-6].fastq.gz 4 biological replicates of paired-end RNA-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
  • ChIP-seq:
    • lane1_MPZezh2WT-24hpf-Ezh2__R[12].fastq.gz: 1 sample of paired-end Ezh2 ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • lane1_MPZezh2WT-24hpf-Rnf2__R[12].fastq.gz: 1 sample of paired-end Rnf2 ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • lane1_MPZezh2WT-24hpf-H3K27me3__R[12].fastq.gz: 1 sample of paired-end H3K27me3 ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • *MPZezh2WT-24hpf-H3K4me3*: 2 biological replicates of paired-end H3K4me3 ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • MPZezh2WT-24hpf-Ezh2-spikein-13277_R[12].fastq.gz: 1 sample of paired-end Ezh2 ChIP-seq data (with Drosophila H2Ay spike in) from 24hpf wild-type (TU/TL background) whole embryo lysates
    • MPZezh2WT-24hpf-H3K27A[cC]*: 2 biological replicates of paired-end H3K27ac ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • MPZezh2WT-24hpf-H3K27me3-spikein-13275_R[12].fastq.gz: 1 sample of paired-end H3K27me3 ChIP-seq data (with Drosophila H2Ay spike in) from 24hpf wild-type (TU/TL background) whole embryo lysates

Notes

The work was funded by the Innovative Research scheme of the Netherlands Organisation for Scientific research (www.nwo.nl, NOW-Vidi 864.12.009, L.M.K.), the Radboud University Nijmegen Medical Centre tenure track fellowship (www.radboudumc.nl, L.M.K.), the Howard Hughes Medical Institute and the Huntsman Cancer Institute core facilities (CA24014, B.R.C.), and the the Eunice Kennedy Shriver National Institute of Child Health and Human Development of the NIH (T32HD007491, P.J.M.).

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