Published September 21, 2023
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TABLE 1 in Endophytic fungi associated with Coriaria nepalensis in an abandoned open-pit phosphate mine in Yunnan, P. R. China
Authors/Creators
- 1. College of Resources and Environment, Yunnan Agricultural University, Kunming, Yunnan 650201, People's Republic of China & lym 2002 @ foxmail. com; https: // orcid. org / 0009 - 0001 - 8348 - 1254
- 2. Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, People's Republic of China & Environmental Science Research Center: Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand & erfu 20170431 @ gmail. com; https: // orcid. org / 0000 - 0003 - 2385 - 6402
- 3. College of Resources and Environment, Yunnan Agricultural University, Kunming, Yunnan 650201, People's Republic of China & daochengjiao @ gmail. com; https: // orcid. org / 0009 - 0007 - 1111 - 861 X
- 4. College of Resources and Environment, Yunnan Agricultural University, Kunming, Yunnan 650201, People's Republic of China & 2792054624 @ qq. com; https: // orcid. org / 0009 - 0009 - 7488 - 9643
- 5. Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, People's Republic of China & National Institute of Fundamental Studies (NIFS), Hantana Road, Kandy, Sri Lanka & samanthakarunarathna @ gmail. com; https: // orcid. org / 0000 - 0001 - 7080 - 0781
- 6. Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, People's Republic of China & saowaluckfai @ gmail. com; https: // orcid. org / 0000 - 0002 - 4706 - 6547
- 7. College of Resources and Environment, Yunnan Agricultural University, Kunming, Yunnan 650201, People's Republic of China & ecoyankai @ 126. com; https: // orcid. org / 0009 - 0004 - 1872 - 9884
Description
TABLE 1. The PCR conditions and the primers used in this study.
| Locus | Definition | Primer | Annealing (PCR) | References |
|---|---|---|---|---|
| ITS4 | ||||
| ITS | Internal transcribed spacer | a55 °C, 50 sb | White et al. (1990) | |
| ITS5 | ||||
| LR0R | ||||
| LSU | Large subunit 28S | a55 °C, 50 sb | Vilgalys & Hester (1990) | |
| LR5 | ||||
| 5F | ||||
| rpb2 | DNA-dependent RNA polymerase II largest subunit | a57 °C, 55 sb | Liu et al. (1999) | |
| 7cR | ||||
| 728F | ||||
| tef1-α | Translation elongation factor 1 alpha | a56 °C, 50 sb | Zhang et al. (2021) | |
| 986R | ||||
| Bt2a | ||||
| Bt2b | O’Donnell & Cigelnik | |||
| tub2 | Beta-tubulin 2 | a52 °C, 50 sb | (1997), | |
| T1 | Nosratabadi et al. (2018) | |||
| T22 | ||||
a Initial denaturation of 2 min at 94 °C, followed by 35 cycles of denaturation at 95 °C for 30 sec, annealing temperature and time for different genes are mentioned in Table 1. b 90 sec of extension at 72 °C and a final extension of 10 minutes at 72 °C.
Notes
Published as part of Lei, Yiming, Yang, Erfu, Dao, Chengjiao, Li, Xi, Karunarathna, Samantha C., Tibpromma, Saowaluck & Yan, Kai, 2023, Endophytic fungi associated with Coriaria nepalensis in an abandoned open-pit phosphate mine in Yunnan, P. R. China, pp. 29 in Phytotaxa 616 (1) on page 29, DOI: 10.11646/phytotaxa.616.1.2, http://zenodo.org/record/8389157
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Related works
- Is part of
- Journal article: 10.11646/phytotaxa.616.1.2 (DOI)
- Journal article: urn:lsid:plazi.org:pub:8631DB131D5AFFA707260B4D3223F97F (LSID)
- Journal article: http://publication.plazi.org/id/8631DB131D5AFFA707260B4D3223F97F (URL)
- Journal article: http://zenodo.org/record/8389157 (URL)
References
- White, T. J., Bruns, T., Lee, S. J. W. T. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315 - 322. https: // doi. org / 10.1016 / B 978 - 0 - 12 - 372180 - 8.50042 - 1
- Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of bacteriology 172: 4238 - 4246. https: // doi. org / 10.1128 / jb. 172.8.4238 - 4246.1990
- Liu, Y. J., Whelen, S. & Hall, B. D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular biology and evolution 16: 1799 - 1808. https: // doi. org / 10.1093 / oxfordjournals. molbev. a 026092
- Zhang, W., Groenewald, J. Z., Lombard, L., Schumacher, R. K., Phillips, A. J. L. & Crous, P. W. (2021) Evaluating species in Botryosphaeriales. Persoonia 46: 63 - 115. https: // doi. org / 10.3767 / persoonia. 2021.46.03
- Nosratabadi, M., Kachuei, R., Rezaie, S. & Harchegani, A. B. (2018) Beta-tubulin gene in the differentiation of Fusarium species by PCR- RFLP analysis. Le infezioni in medicina 26: 52 - 60. https: // doi. org / 10.1016 / j. fgb. 2019.103251