Published August 19, 2024
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TABLE S 1. PCR protocols for 16 S, 28 S in A new genus of the tribe Blondeliini (Diptera: Tachinidae: Exoristinae) from the Oriental Region with morphological and molecular evidence
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TABLE S1. PCR protocols for 16S, 28S and white amplification.
| DNA polymerase | PCR protocols for 16S and 28S amplification |
|---|---|
| ExTaq | 95℃ for 60 s; 40 cycles of 95℃ for 45 s, 50℃ for 45 s, 72℃ for 60 s; extension at 72℃ for 300 s |
| Gflex | 95℃ for 180 s; 40 cycles of 98℃ for 10 s, 50℃ for 15 s, 68℃ for 60 s; extension at 68℃ for 60 s |
| Number of rounds and DNA polymerase | PCR protocols for white amplification |
| First-round for ExTaq | 95℃ for 60 s; 40 cycles of 95℃ for 45 s, 45℃ for 45 s, 72℃ for 60 s; extension at 72℃ for 300 s |
| First-round for Gflex | 95℃ for 180 s; 40 cycles of 98℃ for 10 s, 45℃ for 15 s, 68℃ for 60 s; extension at 68℃ for 60 s |
| Second-round for ExTaq | 95℃ for 60 s; 40 cycles of 95℃ for 45 s, 55℃ for 45 s, 72℃ for 60 s; extension at 72℃ for 300 s |
| Second-round for Gflex | 95℃ for 180 s; 40 cycles of 98℃ for 10 s, 55℃ for 15 s, 68℃ for 60 s; extension at 68℃ for 60 s |
Notes
Published as part of Huang, Yu-Zen & Tachi, Takuji, 2024, A new genus of the tribe Blondeliini (Diptera: Tachinidae: Exoristinae) from the Oriental Region with morphological and molecular evidence, pp. 170 in Zootaxa 5496 (2) on page 170, DOI: 10.11646/zootaxa.5496.2.1, http://zenodo.org/record/13346500
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- Is part of
- Journal article: 10.11646/zootaxa.5496.2.1 (DOI)
- Journal article: urn:lsid:plazi.org:pub:FFE9FF9FFFC97E13FFB9FF80EF44D335 (LSID)
- Journal article: http://publication.plazi.org/id/FFE9FF9FFFC97E13FFB9FF80EF44D335 (URL)
- Journal article: http://zenodo.org/record/13346500 (URL)
- Journal article: http://zoobank.org/FDF92FEA-A841-4CB0-AE31-FC937007DEC0 (URL)