Published April 17, 2024
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TABLE 1 in Morphology and multigene phylogeny reveal a novel Stagonospora species (Massarinaceae, Dothideomycetes) from Thailand
Authors/Creators
- 1. School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand
- 2. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand
- 3. Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036 - 8561, Japan
- 4. Department of Botany and Microbiology, College of Science, King Saud University, P. O Box 2455, Riyadh, 11451, Kingdom of Saudi Arabia
Description
TABLE 1. The amplified loci, primers, and PCR thermal cycle protocols were used in this study.
| Genes/loci | PCR primers (forward/reverse) PCR conditions | Reference | |
|---|---|---|---|
| LSU | LR0R/LR5 | 95 °C; 5 min (94 °C; 30 s, 55 °C; 50 s, 72 °C; 90 s) × 35 thermal cycles, 72 °C; 10 min | Vilgalys & Hester (1990) |
| ITS SSU | ITS1 / ITS4 NS1 / NS4 | 94 °C; 2 min (95 °C; 30 s, 55 °C; 50 s, 72 °C; 90 s) × 35 thermal cycles, 72 °C; 10 min | White et al. (1990) |
| tef 1-α | EF1-983F / EF1-2218R | 95 °C; 5 min (95 °C; 30 s, 58 °C; 50 s, 72 °C; 1 min) Rehner & Buckley (2005) × 35 thermal cycles, 72 °C; 10 min | |
Notes
Published as part of Bhagya, Amuhenage T., Phukhamsakda, Chayanard, Tanaka, Kazuaki & Jones, E. B. Gareth, 2024, Morphology and multigene phylogeny reveal a novel Stagonospora species (Massarinaceae, Dothideomycetes) from Thailand, pp. 283 in Phytotaxa 644 (4) on page 283, DOI: 10.11646/phytotaxa.644.4.4, http://zenodo.org/record/13214798
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- Is part of
- Journal article: 10.11646/phytotaxa.644.4.4 (DOI)
- Journal article: urn:lsid:plazi.org:pub:FFBCFFA0FF9AFFEEAF2CFFBEFFD31E7F (LSID)
- Journal article: http://publication.plazi.org/id/FFBCFFA0FF9AFFEEAF2CFFBEFFD31E7F (URL)
- Journal article: http://zenodo.org/record/13214798 (URL)
References
- Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238 - 4246. https: // doi. org / 10.1128 / jb. 172.8.4238 - 4246.1990
- White, T. J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, 315 - 322. https: // doi. org / 10.1016 / B 978 - 0 - 12 - 372180 - 8.50042 - 1
- Rehner, S. A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF 1 - α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84 - 98. https: // doi. org / 10.1080 / 15572536.2006.11832842