Published November 5, 2021
| Version v1
Dataset
Open
Table 2 in Molecular investigation and description of Iberozospeum n. gen., including the description of one new species (Eupulmonata, Ellobioidea, Carychiidae)
Authors/Creators
- 1. Natural History Museum Bern, 3005 Bern, Switzerland & Institute of Ecology and Evolution, University of Bern,
- 2. Natural History Museum Bern, 3005 Bern, Switzerland & Institute of Ecology and Evolution, University of Bern, & Senckenberg Research Institute and Natural History Museum, 60325 Frankfurt am Main, Germany
- 3. Departamento de Zoología Y Biología Celular Animal,
Description
Table 2 Primer designs used for PCR reactions
| Gene | Primer | Sequence | Sequence length (bp) | Reference |
|---|---|---|---|---|
| COI | LCO1490 | 5’-GGTC AAC AAATCA TAAAGA TATT GG-3’ | 680 | Folmer et al. (1994) |
| HCO2198 | 5’-TAAACTTCAGGGT GACC AAAAAATC A-3’ | |||
| 16S | 16S ar | 5’-CGC CTG TTT ATC AAA AAC AT-3’ | 440 | Simon et al. (1994) |
| 16S br | 5’- CCG GTC TGA ACT CTG ATC AT -3’ | |||
| H3 | H3AD | 5’-ATGGCTCG TA CCAA GCAGA CVGC-3’ | 380 | Colgan et al. (1998) |
| H3BD | 5’-ATAT CCTTRGGCA TRATRG TGAC-3’ | |||
| ITS2 | ITS2ModA | 5’-GCTTGCGGAG AATT AATGT GAA-3’ | 900 | Bouaziz-Yahiatene et al. (2017) |
| ITS2ModB | 5’-GGTA CCTTGTT CGCTA TCGGA-3’ |
Notes
Published as part of Kneubühler, Jeannette, Jochum, Adrienne, Prieto, Carlos E. & Neubert, Eike, 2022, Molecular investigation and description of Iberozospeum n. gen., including the description of one new species (Eupulmonata, Ellobioidea, Carychiidae), pp. 66 in Organisms Diversity & Evolution 22 (1) on page 66, DOI: 10.1007/s13127-021-00517-9, http://zenodo.org/record/12801053
Files
Files
(1.5 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:717e604ad47d90c45fc123f2df516636
|
1.5 kB | Download |
System files
(6.9 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:e82d5465e702d5c6da7a2d7e8ccaf184
|
6.9 kB | Download |
Additional details
Identifiers
Related works
- Is part of
- Journal article: 10.1007/s13127-021-00517-9 (DOI)
- Journal article: urn:lsid:plazi.org:pub:FFADFFDB1143FF941113E03172387E67 (LSID)
- Journal article: http://publication.plazi.org/id/FFADFFDB1143FF941113E03172387E67 (URL)
- Journal article: http://zenodo.org/record/12801053 (URL)
References
- Folmer, O., Black, M., Hoe, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294 - 299.
- Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H., & Flook, P. (1994). Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87 (6), 651 - 701.
- Colgan, D., McLauchlan, A., Wilson, G. D. F., Livingston, S. P., & Edgecombe, G. D. (1998). Histone H 3 and U 2 snRNA DNA sequences and arthropod molecular evolution. Australian Journal of Zoology, 46, 419 - 437.
- Bouaziz-Yahiatene, H., Pfarrer, B., Medjdoub-Bensaad, F., & Neubert, E. (2017). Revision of Massylaea Mollendorff, 1898 (Stylommatophora, Helicidae). ZooKeys 694, 109 - 133. https: // doi. org / 10.3897 / zookey s. 694.15001