Published December 31, 2020
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Table 3 in New record of Nausithoe werneri (Scyphozoa, Coronatae, Nausithoidae) from the Brazilian coast and a new synonymy for Nausithoe maculata
- 1. Departamento de Zoologia, Instituto de Biociencias, Universidade de Sao Paulo, Rua do Matao, travessa 14, n. 101, Cidade Universitaria, Sao Paulo, SP, 05508 - 090, Brazil
- 2. Departamento de Zoologia, Instituto de Biociencias, Universidade de Sao Paulo, Rua do Matao, travessa 14, n. 101, Cidade Universitaria, Sao Paulo, SP, 05508 - 090, Brazil & Centro de Biologia Marinha, Universidade de Sao Paulo, Rodovia Manuel Hypolito do Rego km 131.5, Sao Sebastiao, SP, 11600 - 000, Brazil
Description
Table 3. List of primers used to amplify each gene. *LEM = Laboratório de Evolução Molecular (IB-USP).
| Gene | Primer | Primer sequence 5'-3' | F/R | Temp. - base pairs | Source |
|---|---|---|---|---|---|
| COI | COXl-F2 | TCGACTAATCATAAAGATATCGGCAC | F | 52 °C - 26 bp | Ward et al. 2005 |
| MEDCOXR | TGGTGNGCYCANACNATRAANCC | R | 52 °C - 23 bp | Lawley et al. 2016 | |
| LCO1490JJ4 | CIACIAAYCAYAARGAYATYGG | F | 55 °C+45 °C - 22 bp | Astrin et al. 2016 | |
| HCO2198JJ4 | ANACTTCNGGRTGNCCAAARAATC | R | 55 °C+45 °C - 25 bp | Astrin et al. 2016 | |
| LCO1490JJ2 | CHACWAAYCAYAARGAYATYGG | F | 60 °C+45 °C - 22 bp | Astrin et al. 2016 | |
| HCO2198JJ2 | ANACTTCNGGRTGNCCAAARAATCA | R | 60 °C+45 °C - 25 bp | Astrin et al. 2016 | |
| 28S | F15 | CTAACAAGGATTCCCCTAGTAACGGCGAG | F | 55.5 °C - 30 bp | LEM * |
| R798 | GGTCCGTGTTTCAAGACGG | R | 55.5 °C - 19 bp | Medina et al. 2001 | |
| F798 | CCGTCTTGAAACACGGACC | F | 55.5 °C - 19 bp | Medina et al. 2001 | |
| R1446 | GTTGTTACACACTCCTTAGCGG | R | 55.5 °C - 22 bp | Medina et al. 2001 | |
| 18S | 18S - A | AACCTGGTTGATCCTGCCAGT | F | 54 °C - 21 bp | Medlin et al. 1988 |
| 18S - L | CCAACTACGAGCTTTTTAACTG | R | 54 °C - 22 bp | Apakupakul et al. 1999 | |
| 18S - C | CGGTAATTCCAGCTCCAATAG | F | 54 °C - 21 bp | Apakupakul et al. 1999 | |
| 18S - Y | CAGACAAATCGCTCCACCAAC | R | 54 °C - 21 bp | Apakupakul et al. 1999 | |
| 18S - O | AAGGGCACCACCAGGAGTGGAG | F | 54 °C - 22 bp | Apakupakul et al. 1999 | |
| 18S - B | TGATCCTTCCGCAGGTTCACCT | R | 54 °C - 22 bp | Medlin et al. 1988 |
Notes
Published as part of Molinari, Clarissa Garbi, Maronna, Maximiliano Manuel & Morandini, Andre Carrara, 2020, New record of Nausithoe werneri (Scyphozoa, Coronatae, Nausithoidae) from the Brazilian coast and a new synonymy for Nausithoe maculata, pp. 1 in ZooKeys 984 on page 1, DOI: 10.3897/zookeys.984.56380
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