Table 4 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates
- 1. * & Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark
Description
Table 4 Total glucosinolate (GSL) levels and profiles of GSLs derived from aromatic amino acids in Planodes virginica, Nasturtium officinale and Rorippa sylvestris. a
| N | Total | SD | PE | BAR | EBAR | 4hEBAR | IM | 4moIM | |
|---|---|---|---|---|---|---|---|---|---|
| 105 | 40 S | 40 R | 139 R | 43 | 48 | ||||
| μmol/g dry wt. | |||||||||
| Planodes virginica (Acc. 1, Kentucky) | |||||||||
| Roots | 3 | 6.8 | 1.8 | 0.06 | 0.05 | 4.3 | n.d. | 1.2 | n.d. |
| Stems | 3 | 8.4 | 3.3 | 0.001 | 0.09 | 6.5 | n.d. | 0.62 | n.d. |
| Leaves | 3 | 15 | 4 | 0.001 | 0.18 | 13 | tr. | 1.4 | n.d. |
| Siliques | 3 | 14 | 5 | 0.002 | 0.17 | 11 | n.d. | 0.65 | n.d. |
| Seeds | 1 | 66 | – | 0.14 | 0.73 | 44 | 0.25 | 0.11 | n.d. |
| Planodes virginica (Acc. 2, Ohio) | |||||||||
| Leaves | 5 | 24 | 3 | 0.002 | 0.34 | 17 | (0.03)b | 3.7 | n.d. |
| Nasturtium officinale (two accessions, ‘Wild Ohio’ and ‘Johnny’ s Select Seeds’) | |||||||||
| Seeds (wild Ohio) | 1 | 47 | – | 31 | 0.01* | 0.07 | n.d. | tr.* | tr.* |
| Seeds (Johnny’ s) | 1 | 78 | – | 58 | 0.01* | 0.18 | n.d. | tr.* | tr.* |
| Rorippa sylvestris | |||||||||
| Seeds | 2 | 71 | 26 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
N is number of replicates, SD is standard deviation, other data are mean levels.
a n-HomoMet-derived and other aliphatic GSLs as specified in Table 5. Searched for but not detected (all three species): The typical Barbarea seed isoferuloyl derivatives (6’iF PE, 6’iF IM, 6’iF BAR, 6’iF EBAR), as well as hypothetic isoferuloyl and benzoyl derivatives of other dominating GSLs. Searched for but not detected in N. officinale: BZ, 4hBZ, 4hEBAR.Searched for but not detected in P. virginica and R. sylvestris: all substituted IMs: 4hIM, 1moIM, 4moIM, 1,4moIM. Searched for and not detected in P. virginica: BZ, 4hBAR, hydroxy-methoxy derivatives 4moEBAR and 3/4 hmoEBAR (“ x1 ”). GSL data with an asterisk indicate lack of MS2 confirmation of analyte identity due to low level.
b Confirmed by UHPLC-QToF (Supplementary Fig. S4) and quantified tentatively based on N 3 samples.
Notes
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