Published December 31, 2019 | Version v1

Table 1. Cytochrome c oxidase subunit I in Revision of Claraeola (Diptera, Pipunculidae) in the Middle East based on morphology and DNA barcodes

Description

Table 1. Cytochrome c oxidase subunit I mitochondrial gene primers.

Gene name/regionForward primer nameForward primer sequence (5 ’-3’)Primer referenceReverse primer nameReverse primer sequence (5 ’-3’)Primer reference
COI BarcodeLCO1490GGTCAACAFolmer et al. (1994)COI-Dipt-2183RCCAAAAAATCGibson et al. (2011)
AATCATAAAARAATARRTG
GATATTGGYTG
COI-Fx-A (5' end of barcode)LCO1490GGTCAACAFolmer et al. (1994)COI-SYR-1762RCGDGGRAADYoung et al. (in prep.)
AATCATAAAGCYATRTCDGG
GATATTGG
COI-Fx-B (middle of barcode)COI-SYR-342FGGDKCHCCYoung et al. (in prep.)COI-SYR-1976RGWAATRAARTYoung et al. (in prep.)
NGAYATRGCTWACDGCHCC
COI-Fx-C (3' end of barcode)COI-SYR-1957FGGDATWTCYoung et al. (in prep.)COI-Dipt-2183RCCAAAAAATCAGibson et al. (2011)
HTCHATYYTAGGRAATARRTGYTG

Notes

Published as part of Motamedinia, Behnam, Skevington, Jeffrey H. & Kelso, Scott, 2019, Revision of Claraeola (Diptera, Pipunculidae) in the Middle East based on morphology and DNA barcodes, pp. 85 in ZooKeys 873 on page 85, DOI: 10.3897/zookeys.873.36645

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Additional details

References

  • Folmer, O, Black, M, Hoeh, W, Lutz, R, Vrijenhoek, R, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. . Moelecular Marine Biology and Biotechnology 3: 294 - 299
  • Gibson, JF, Kelso, S, Jackson, MD, Kits, JH, Miranda, GFG, Skevington, J, 2011. Diptera-Specific Polymerase Chain Reaction Amplification Primers of Use in Molecular Phylogenetic Research. . Annals of the Entomological Society of America 104: 976 - 997