Published December 31, 2019
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Table 1. Cytochrome c oxidase subunit I in Revision of Claraeola (Diptera, Pipunculidae) in the Middle East based on morphology and DNA barcodes
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Description
Table 1. Cytochrome c oxidase subunit I mitochondrial gene primers.
| Gene name/region | Forward primer name | Forward primer sequence (5 ’-3’) | Primer reference | Reverse primer name | Reverse primer sequence (5 ’-3’) | Primer reference |
|---|---|---|---|---|---|---|
| COI Barcode | LCO1490 | GGTCAACA | Folmer et al. (1994) | COI-Dipt-2183R | CCAAAAAATC | Gibson et al. (2011) |
| AATCATAAA | ARAATARRTG | |||||
| GATATTGG | YTG | |||||
| COI-Fx-A (5' end of barcode) | LCO1490 | GGTCAACA | Folmer et al. (1994) | COI-SYR-1762R | CGDGGRAAD | Young et al. (in prep.) |
| AATCATAAA | GCYATRTCDGG | |||||
| GATATTGG | ||||||
| COI-Fx-B (middle of barcode) | COI-SYR-342F | GGDKCHCC | Young et al. (in prep.) | COI-SYR-1976R | GWAATRAART | Young et al. (in prep.) |
| NGAYATRGC | TWACDGCHCC | |||||
| COI-Fx-C (3' end of barcode) | COI-SYR-1957F | GGDATWTC | Young et al. (in prep.) | COI-Dipt-2183R | CCAAAAAATCA | Gibson et al. (2011) |
| HTCHATYYTAGG | RAATARRTGYTG |
Notes
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References
- Folmer, O, Black, M, Hoeh, W, Lutz, R, Vrijenhoek, R, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. . Moelecular Marine Biology and Biotechnology 3: 294 - 299
- Gibson, JF, Kelso, S, Jackson, MD, Kits, JH, Miranda, GFG, Skevington, J, 2011. Diptera-Specific Polymerase Chain Reaction Amplification Primers of Use in Molecular Phylogenetic Research. . Annals of the Entomological Society of America 104: 976 - 997