Published January 31, 2021
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Table 1 in Probing of the plasticity of the active site in pinene synthase elucidates its potential evolutionary mechanism
Description
Table 1 Major products (%±SD) of WT α- pinene synthase and enzymes with mutations.
| Enzymes | Relative Proportion of Products (%) | ||||||
|---|---|---|---|---|---|---|---|
| Unknown | α- pinene | sabinene | limonene | Unknown | Unknown | Activity(% WT) | |
| RT = 4.52 | RT = 7.05 | RT = 8.55 | RT = 11.05 | RT = 11.58 | RT = 12.72 | ||
| PS-WT | 0.31 ± 0.02 | 97.86 ± 0.21 | 0.96 ± 0.00 | 0.26 ± 0.01 | 0.54 ± 0.05 | 0.07 ± 0.00 | 100 |
| F482A | 0.05 ± 0.00 | 4.43 ± 0.03 | 93.21 ± 0.06 | 1.59 ± 0.05 | 0.72 ± 0.03 | 0.00 ± 0.00 | 46.63 ± 4.47 |
| S491A | 0.46 ± 0.01 | 97.28 ± 0.09 | 1.14 ± 0.00 | 0.12 ± 0.00 | 0.99 ± 0.06 | 0.00 ± 0.00 | 128.90 ± 4.19 |
| F482I | 1.27 ± 0.02 | 3.17 ± 0.08 | 92.35 ± 0.21 | 0.66 ± 0.09 | 2.33 ± 0.08 | 0.23 ± 0.00 | 18.36 ± 2.51 |
| F482L | 0.96 ± 0.06 | 2.03 ± 0.03 | 94.26 ± 0.30 | 0.31 ± 0.01 | 1.91 ± 0.17 | 0.29 ± 0.01 | 49.98 ± 39.78 |
| F482V | 1.03 ± 0.01 | 2.12 ± 0.03 | 94.51 ± 0.07 | 0.30 ± 0.03 | 1.82 ± 0.06 | 0.23 ± 0.00 | 29.50 ± 12.39 |
| F482T | 0.00 ± 0.00 | 3.53 ± 0.00 | 93.91 ± 0.00 | 1.64 ± 0.04 | 0.92 ± 0.05 | 0.00 ± 0.00 | 52.69 ± 3.17 |
| F482Y | 0.00 | 2.71 | 31.00 | 63.61 | 2.68 | 0.00 | 0.62 |
| F482R | 17.62 ± 6.21 | 0.00 ± 0.00 | 18.96 ± 7.19 | 31.04 ± 19.27 | 27.33 ± 14.94 | 5.05 ± 0.51 | 1.28 ± 0.01 |
| F482W | 16.32 ± 5.33 | 0.00 ± 0.00 | 29.92 ± 17.90 | 21.67 ± 6.84 | 27.35 ± 14.96 | 4.74 ± 0.45 | 1.54 ± 0.02 |
| I335A | 3.52 ± 0.02 | 78.00 ± 0.03 | 12.94 ± 0.00 | 0.57 ± 0.02 | 4.28 ± 0.03 | 0.67 ± 0.00 | 54.36 ± 2.17 |
| F482A/S491A | 1.34 ± 0.11 | 4.17 ± 0.01 | 88.40 ± 1.43 | 3.38 ± 0.14 | 2.36 ± 0.34 | 0.35 ± 0.01 | 18.73 ± 0.13 |
| F482I/S491A | 0.63 ± 0.02 | 2.72 ± 0.00 | 92.88 ± 0.45 | 2.40 ± 0.09 | 1.19 ± 0.08 | 0.18 ± 0.00 | 28.74 ± 5.73 |
Notes
Published as part of Xu, Jingwei, Peng, Guanzu, Xu, Jinkun, Li, Yi, Tong, Li & Yang, Dong, 2021, Probing of the plasticity of the active site in pinene synthase elucidates its potential evolutionary mechanism, pp. 3 in Phytochemistry (112573) 181 on page 3, DOI: 10.1016/j.phytochem.2020.112573, http://zenodo.org/record/8291066
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