Nikon GA3 Recipe for AI-Assisted Immunofluorescence Image Analysis in Human Enteric Neurons

This dataset contains a Nikon GA3 analysis recipe designed for automated segmentation and quantification of immunofluorescence images of human enteric neurons. Utilizing Nikon's NIS-Elements software with AI-powered image processing, the recipe enables accurate fluorescence intensity quantification and neuron volume segmentation. The workflow minimizes manual bias, enhances reproducibility, and provides quantitative insights into neuronal marker colocalization. This tool is particularly useful for researchers studying enteric nervous system structure and function in health and disease.