Published March 12, 2025 | Version v1
Dataset Open

Microscopy and raw data for Alkane 1-monooxigenase gene alkB of Pseudomonas sp. FF2 is upregulated during colonisation of Arabidopsis thaliana leaves

Authors/Creators

  • 1. EDMO icon Freie Universitaet Berlin
  • 2. ROR icon Freie Universität Berlin
  • 3. University of Canterbury

Description

This dataset accompanies the analysis of bacterial single-cell fluorescence and colony-forming unit (CFU) measurements in both in vitro and in planta conditions. The study focuses on the bacterial strain Pseudomonas fluorescens PFF2, evaluating its growth and promoter activity of the alkB gene using fluorescence under different growth conditions (diesel supplement growth medium and the Arabidopsis thaliana phyllosphere). The dataset supports machine learning classification models for bacterial cell identification and statistical analyses.

Codes are available at https://github.com/relab-fuberlin/alkanebioreporter

File description:

File Data type Description
invitro.tar.gz Microscopy images Images of in vitro experiments (PFF2 grown in BHB with and without diesel, and in LB)
inplanta-rep1.tar.gz Microscopy images Images of planta experiment 1 (PFF2 grown on Arabidopsis thaliana at 0, 2 and 7 dpi)
inplanta-rep2.tar.gz Microscopy images Images of planta experiment 2 (PFF2 grown on Arabidopsis thaliana at 0, 2 and 7 dpi)
in_vitro_rawdata.zip CSV CFU and single-cell fluorescence data of in vitro experiments
in_planta_rawdata.zip CSV CFU and single-cell fluorescence data of in planta experiments
trainset.zip CSV Ground truth-label dataset for training classification models

Files

in_planta_rawdata.zip

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