Lactic Acid can Directly Bind Genes and Alter Transcription within Ovaries

Supplementary dataset 1

Related to Fig. 2 and Supplementary Fig. 1A-F. This file includes 16 sheets (from left to right):

Sheet “1.1-all LABP” includes all LABP from Bio-LA IP-mass spec, Sheet “1.2-LABP-Log2FC>1” are LABP with Log2 FC (Bio-LA/Biotin only) > 1.

Sheet “2.1-all SABP” includes all SABP from Bio-SA IP-mass spec, Sheet “2.2-SABP-Log2FC>1” are SABP with Log2 FC (Bio-SA/Biotin only) > 1.

Sheet “3.1-all BABP” includes all BABP from Bio-BA IP-mass spec, Sheet “3.2-BABP-Log2FC>1” are BABP with Log2 FC (Bio-BA/Biotin only) > 1.

Sheet “4.1-all lactylated” includes all lactylated proteins from anti-lactylated lysine Ab IP-mass spec, Sheet “4.2-lactylated-Log2FC>1” are lactylated proteins with Log2 FC (lactylated/control IgG) > 1.

Sheet “5.1-all succinylated” includes all succinylated proteins from anti-succinylated lysine Ab IP-mass spec, Sheet “5.2-succinylated-Log2FC>1” are succinylated proteins with Log2 FC (lactylated/control IgG) > 1.

Sheet “6.1-all butyrylated” includes all butyrylated proteins from anti- butyrylated lysine Ab IP-mass spec, Sheet “6.2-butyrylated-Log2FC>1” are butyrylated proteins with Log2 FC (butyrylated /control IgG) > 1.

Sheet “7-net LABP-(1.2 minus 4.2)” are LABP (Log2 FC > 1) in sheet 1.2 substracted by lactylated proteins in sheet 4.2, which will be LA-binding-only but not lactylated proteins.

Sheet “8-net SABP-(2.2 minus 5.2)” are SABP in sheet 2.2 substracted by succinylated proteins in sheet 5.2, which will be SA-binding-only but not succinylated proteins.

Sheet “9-net BABP-(3.2 minus 6.2)” are BABP in sheet 3.2 substracted by butyrylated proteins in sheet 6.2, which will be BA-binding-only but not butyrylated proteins.

Sheet “10-all CABP” includes all CABP from Bio-CA IP-mass spec.

LABP, LA-binding proteins; SABP, SA-binding proteins; BABP, BA-binding proteins; CABP, CA-binding proteins.

Supplementary dataset 2

Related to Fig. 3. This file includes 8 sheets. “1.1-All LA-binding regions” includes all ChIP seq DNA fragments with LA encrichment at the threshold of FE (Bio-LA/Biotin only) > 1.5; while in “1.2-All LA-binding genes” sheet, a highest FE value among all identified DNA fragments (in “1.1-All LA-binding regions” sheet) of the same gene were used as the representative FE of the gene. The rest sheet couples (2.1 and 2.2; 3.1 and 3.2; 4.1 and 4.2) follow the same principle except that the metabolites are SA, BA, CA respectively.

Supplementary dataset 3

Related to Fig. 4. This file includes 1 sheets. “All genes-LA vs control” includes all expression information in LA-treated or control ovaries.

Supplementary dataset 4

Related to Fig. 5. This file includes 3 sheets. “All LABG-FE>1” includes all LA-enriched genes from ChIP seq with FE (Bio-LA/Biotin only) > 3; “All DEGs-FC>1.5 or <0.67” includes all DEGs with FC (LA-treated/control) > 1.5 or < 0.67; “”Overlapping-LABG vs DEGs” included genes that are identified both in “All LABG-FE>3” and in “All DEGs-FC>1 or <0.67”.