Behaviour and Reproduction of Drosophila Melanogaster Exposed to 3.5 GHz Radio-Frequency Electromagnetic Fields - Underlying data
Authors/Creators
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1.
University of Southampton
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2.
Ghent University
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3.
City University of New York
Description
Insects are exposed to radio-frequency electromagnetic fields emitted by wireless telecommunication networks. A part of these fields will be absorbed inside of these insects. This absorption might have biological effects, depending on the amount of absorbed power. It is currently unknow at what level of absorption this might occur. To investigate this, we used RF dosimetry of adult D. melanogaster flies, which we combined with two assays studying the locomotor activity and fecundity of D. melanogaster exposed to electromagnetic fields at 3.6 GHz. To perform dosimetry, we created a 3D digital model of an adult fly using micro-CT scans of a female D. melanogaster. We used this model in numerical EM simulations to estimate the absorbed power in the fly as a function of RF frequency in the far field of an antenna and during the two experimental assays at 3.6 GHz. No effects were found on locomotor activity for a five-day exposure to 3.6 GHz between 5.4 and 9 V/m, which correspond to 3.56 nW to 9.88 nW of absorbed power. We also did not find any effects on fecundity, at an absorption level of 1.91 mW during 48h at 3.6 GHz during 48h. In our future work, we aim to investigate this exposure at higher frequencies and for immature stages as well.
Table of contents
Overview
This dataset contains segmented STL 3D models, TIFF imaging data, and visualisations of the Drosophila (fruit fly) scan used in this study
File Index
STL 3D Models (Drosophila.zip)
These files represent different anatomical structures of Drosophila in STL format for 3D visualization, printing, and analysis.
| File Name | Description | File Size |
|---|---|---|
| Drosophila_brain.stl | 3D model of the fruit fly brain | 9.79 MB |
| Drosophila_exoskeleton.stl | External structure/exoskeleton | 42.6 MB |
| Drosophila_gonads.stl | Reproductive organs | 42.9 KB |
| Drosophila_halteres.stl | Small hind-wing balancing organs | 2.55 MB |
| Drosophila_internal_tissue.stl | Internal soft tissues | 10.6 MB |
| Drosophila_muscles.stl | Muscle structures | 1.71 MB |
| Drosophila_wing_left.stl | Left wing structure | 2.07 MB |
| Drosophila_wing_right.stl | Right wing structure | 2.11 MB |
Metadata
Name: Drosophila melanogaster- Blue Bottle Fly - Adult
Order: Diptera
Family: Drosophilidae
Species: Drosophila melanogaster
Stage: adult - female
Origin: Reared in the lab of Herman Wijnen at University of Southampton, UK, in 2022
Classification: 2022 by Herman Wijnen of University of Southampton, UK, in 2022
Preservation: Following 5 min incubation in 0.5% Triton X-100 PBS, the flies were fixed in Bouin’s solution for 24h, washed in 1x PBS and then stained for 2d in Lugol’s solution prior to washing in 1x PBS and scanning in ultrapure water.
Scanning: Done by dr. Orestis Katsamenis using a custom Nikon XTH225ST μCT system. The X-ray tube was operated at 80 kVp and 36 μA, delivering a power of 2.88 W.
TIFF Imaging Data
These TIFF files contain μCT -based X-ray histology (XRH) scans of the Drosophila specimen.
| File Name | Description | File Size |
|---|---|---|
| 20221110_XRH_3175_OLK_MBIO07123-FIXED__0001.tif | Individual XRH image slice | 4.1 MB |
| 20221110_XRH_3175_OLK_MBIO07123-FIXED__0250.tif | Individual XRH image slice | 4.1 MB |
| 23330_20221110_XRH_3175_OLK_MBIO07123-FIXED_692x960x1152x16bit.tif | Full Drosophila volume dataset | 1.5 GB |
The .png files are artistic renderings of average and standard deviation projections, mapped as different color channels.
| File Name | Description | File Size |
|---|---|---|
| Composite_1.png | Composite projection rendering | 897.9 kB |
| Composite_2.png | Composite projection rendering | 897.9 kB |
| Composite_3.png | Composite projection rendering | 1.1 MB |
| Composite_4.png | Composite projection rendering | 1.0 MB |
| Montage-5x2.png | Montage of 10 axial slices across Drosophila | 182.5 kB |
Compressed Archives
These .zip files contain multiple dataset components for easier distribution.
| File Name | Description | File Size |
|---|---|---|
| Drosophila.zip | Archive containing all STL files listed above | 39.3 MB |
| tiffStackx16bit.zip | Archive containing TIFF imaging data (stacked slices) | 854.4 MB |
Usage
- STL models can be opened in MeshLab, Blender, 3D Slicer, Paraview, etc
- TIFF imaging data can be analysed using ImageJ, Fiji, Avizo, or 3D Slicer, etc
- Montage-5x2.png provides an overview of axial slices across the specimen.
Attribution
If using these files in research, please cite the appropriate sources.
Files
Composite_1.png
Files
(2.4 GB)
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854.4 MB | Preview Download |
Additional details
Related works
- Is supplemented by
- Dataset: https://skfb.ly/pu8V9 (URL)