Published February 7, 2025 | Version v1

Staphylococci Knockout Screening

Description

Sample Genotype Detail
Tryptic soy broth (TSB)   Blank standard growth media control
AH5011 S. hominis D11 (wildtype) Postive control / Bacteriocin-producing strain
AH6424 S. hominis AH5011 ∆pHom∆pMup Plasmid-cured mutant
AH0203 S. aureus RN4220 (wildtype) Negative control
AH6425  S. aureus RN4220 pCM28-HomLocus Reengineered S. aureus-expressing bacteriocin
AH6433 S. aureus RN4220 pCM28-empty vector Negative control / Empty vector control
AH6434 S. aureus RN4220 pCM28-HomLocus-∆homI Reengineered S. aureus-expressing bacteriocin without the immunity protein, HomI
AH6447 S. aureus RN4220 pCM28-HomLocus-∆homBCDM Reengineered S. aureus-expressing bacteriocin without the biosynthesis genes: HomB, HomC, HomD2, and HomM2
D11_Peptide Purified bacteriocin  

1 , 2, 3 - Injection Replicates

Files

Files (4.2 GB)

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