Dataset for STAR Protocols for NanoNm to detect 2'-O-methylation in rRNA of fly, yeast and human, in addition to the human mRNA.

This dataset contains rRNA sequences and Nm position information for human, yeast, and fly rRNAs. Additionally, the toy example dataset includes FAST5 files for mRNA and rRNA from prostate cancer C4-2 cells.

The feature dataset for human, yeast, and fly rRNAs has been pre-processed and is used in the examples provided in the STAR Protocols.

git clone https://github.com/kaifuchenlab/STAR_Protocol   

./genome: The folder used to store genome and annotation files for the Nm detection in mRNA.

./raw_data: The folder used to store the raw data of fast5 files downloaded for the processing.

./example_data: The toy fast5 files of human mRNA and rRNA used for the STAR protocols.

./rRNA: The rRNA information for human, yeast and fly.

./scripts: Custom scripts for STAR protocols from https://github.com/kaifuchenlab/STAR_Protocol/tree/main/scripts.

./Yeast_Fly_feature_dataset: The processed feature dataset for yeast and fly.