COMPARATIVE STUDY OF TRADITIONAL FORMALDEHYDE VERSUS FORMALDEHYDE FREE- SATURATED SALT AS EMBALMING SOLUTIONS FOR GROSS ANATOMY AND SURGICAL TRAINING OF STUDENTS

The aim of this study was to compare the chemical and microbiological effects of two preservatives used for the embalming of cadavers for teaching of veterinary students in anatomy laboratory. Twelve cats recommended for euthanasia were included in this study. All the cats (n=12) were divided into two treatment groups (n=6 each). In Group A, 10 % formaldehyde was used with phenol, glycerin and water. In Group B Saturated Salt was used with phenol, glycerin and water. Microbiological examination was performed and evaluated by pour plate technique. The range of motion of the joints and freshness was evaluated by Goniometer and Konica Minolta C-400 Chroma Meter respectively. Data thus obtained were compared between groups using paired T-test. Differences at (P<0.05) were considered significant. Cats administered with saturated salt solution showed reduction in bacterial and mold growth, joints flexibility, retained their natural consistency and color than traditional formaldehyde treated group (P>0.05). The range of motion of joints at day 20th, 40th and 60th were significantly higher (P <0.05) in group B as compared to group A. The muscle consistency and color was graded as L*, a*, b*, C* and h* and their values were (65.21±0.50, 6.17±0.20, 12.79±0.31, 11.03±0.25 and 30.71±0.35) and (58.74±0.35, 8.22±0.20, 9.59±0.27, 13.08±0.20 and 25.36±0.22) in formaldehyde and SSS groups respectively. The responses of DVM students were positive for irritation of eyes, sore throat, coughing and bad smell in formaldehyde group. In conclusion, the SSS method is simple and cheap with a low risk of infection as compared to formaldehyde.

Keywords:  Embalming; Veterinary Gross anatomy and surgery; Saturated salt solution (SSS); formaldehyde 10%.