Example Files for pRESTO Stern JNH, Yaari G, and Vander Heiden JA, et al. 2014

This example uses publicly available data from:

B cells populating the multiple sclerosis brain mature in the draining cervical lymph nodes.
Stern JNH, Yaari G, and Vander Heiden JA, et al.
Sci Transl Med. 2014. 6(248):248ra107. doi:10.1126/scitranslmed.3008879.

Which may be downloaded from the NCBI Sequence Read Archive under BioProject accession ID: PRJNA248475. For this example, we will use the first 25,000 sequences of sample M12 (accession: SRR1383456), which may downloaded downloaded using fastq-dump from the SRA Toolkit:

fastq-dump --split-files -X 25000 SRR1383456

Primers sequences are available online at the supplemental website for the publication.