Data for the article LOM-24-05-0051 published in Limnology and Oceanography Methods by Mellilti et al

Reference : LOM-24-05-0051 

Title : Quantifying invasion dynamics: qPCR vs. ddPCR in monitoring the alien invasive bloody-red shrimp Hemimysis anomala and its interaction with perch in Lake Geneva

Authors : Sirine Melliti, Marine Vautier, Cécile Chardon, Stéphan Jacquet

Correspondence : Stéphan Jacquet, UMR CARRTEL, 75bis Avenue de Corzent, 74203 Thonon-les-Bains Cedex, France. E-mail: stephan.jacquet@inrae.fr

This file is composed by 3 different sheets 

"DNA concentration" sheet: 

- Calculations to get, from copies/μL reaction, copies/Litre of filtered water for Hemimysis anomala and Perca fluviatilis for ddPCR

- Concentrations in copies/L of filtered water for Hemimysis anomala for qPCR 

"qPCR vs ddPCR" sheet:

Log Hemimysis anomala DNA concentrations (copies/L filtered water) for qPCR and ddPCR 

"LOD-LOQ" sheet:

Calculations of the Limits of Detection and Quantitation for the ddPCR based on a range of 8 successive concentrations of Hemimysis anomala with 5 replicates

-          LOD (the lowest concentration at which the target DNA was detected in at least one replicate)

-          LOQ (the lowest concentration with a coefficient of variation value of less than 35%)