OmniSegger - Figure data files

Proliferation dataset: OmniSegger output for phase-contrast images of wild-type MG1655 E. coli grown on a 4% agarose pad. Frame rate: 3min/frame. 

Morphology dataset: OmniSegger output for phase-contrast images of wild-type MG1655 E. coli treated with 10uM hydroxyurea growing on a 2% agarose pad. Frame rate: 5min/frame. 

Modality dataset-brightfield under-focused: OmniSegger output for under-focused brightfield and fluorescence images of TB28 attHK022 Plac::zipA-sfGFP bla pal-mCherry cat E. coli growing on a 2% agarose pad. Images chosen from 0.5um below the focal plane. Frame rate:  15min/frame.  

Modality dataset-brightfield over-focused: OmniSegger output for over-focused brightfield and fluorescence images of TB28 attHK022 Plac::zipA-sfGFP bla pal-mCherry cat E. coli growing on a 2% agarose pad. Images chosen from 0.5um above the focal plane. Frame rate: 15min/frame. 

Modality dataset-cytoplasmic fluorescence: OmniSegger output for phase-contrast and fluorescence images of lysC-GFP fusion E. coli strain from the ASKA collection growing on a 2% agarose pad. Frame rate: 7min/frame.   Fatal errors calculated by Omnipose bact_fluor_omni segmentation on fluorescence images only, files available upon request.

Modality dataset-membrane fluorescence: OmniSegger output for phase-contrast and fluorescence images of ygaW-GFP fusion E. coli strain from the ASKA collection growing on a 2% agarose pad. Frame rate: 7min/frame.  Fatal errors calculated by Omnipose bact_fluor_omni segmentation on fluorescence images only, files available upon request.

Images taken in the Wiggins Lab at UW. Imaging was performed using a Nikon Eclipse Ti-E microscope, through a 60X 1.4 NA CFI oil-immersion Phase objective onto an Andor Neo sCMOS camera.