MinION 1D² Reads From Mus musculus GL261 Cell Lines

Called FASTQ and raw FAST5 MinION cDNA reads (1D²) from a murine GL261 neuroblastoma cell line, cultured at the Malaghan Institute of Medical Research, sequenced on a R9.5 flow cell in August 2017 using the LSK309 1D² kit for ligating ONT adapters to cDNA generated using strand-switching primers.

The called reads for the entire sequencing run are available:

• called_reads_1Dsq_Olivier_GL261_cDNA_2017-Aug-04.tar.gz -- called reads from both/all runs (1D²-corrected fastq files only).
• called_reads_uncorrected_Olivier_GL261_cDNA_2017-Aug-04.tar.gz -- uncorrected reads from both/all runs.
• metadata_called_reads_Olivier_GL261_cDNA_2017-Aug-04.tar.gz -- metadata associated with all called sequences (e.g. sequencing_summary.txt)

This dataset only includes a subset of the total reads as raw signal / FAST5 files:

• Actb_GL261_cDNA_2017-Aug-04_1D2.tar -- reads from one run that mapped (in whole or in part) to a mouse beta-actin transcript [ENSMUST00000100497.10].
• Ubb_GL261_cDNA_2017-Aug-04_1D2.tar -- reads from one run that mapped (in whole or in part) to a mouse ubiquitin transcript [ENSMUST00000019649.3].