MASLD metabolomics -- SWATH -- HILIC -- Metabolomics -- Positive mode
Authors/Creators
- 1. Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
- 2. Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore
- 3. Cardiovascular & Metabolic Disease Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Description
We analyzed liver tissues, liver secretome (conditioned medium), and blood plasma samples from 109 obese patients undergoing bariatric surgery spanning early stages of metabolic dysfunction-associated steatotic liver diseases (MASLD). We used a combination of data-dependent acquisition (DDA) and data-independent acquisition (DIA or SWATH-MS) on SCIEX TripleTOF 6600.
Metabolites and lipids were extracted using modified chloroform-methanol biphasic extraction methods for both biological matrices. Organic and aqueous fractions were separated using RRHD Eclipse Plus C18 and SeQuant ZIC-cHILIC columns prior to DDA and DIA acquisitions, respectively.
All mzML files are deposited with ZENODO.
*** HILIC - Polar metabolite - Positive mode - SWATH is deposited in this dataset repository ***
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- Dataset: 10.5281/zenodo.14091962 (DOI)