Dataset Open Access

Construction, validation and application of nocturnal pollen transport networks in an agro-ecosystem: datasets collected using light microscopy and DNA metabarcoding

Macgregor, Callum J; Kitson, James JN; Fox, Richard; Hahn, Christoph; Lunt, David H; Pocock, Michael JO; Evans, Darren M

This dataset contains all data required to reproduce the analyses conducted in Macgregor et al. (2018), using the R Notebook archived at doi: 10.5281/zenodo.1322712.

Specifically, the dataset contains details of pollen transport detected on two matched samples, each containing 311 moths of 41 species, using two methods: a traditional light microscopy approach and a novel DNA metabarcoding approach. Both raw and manually-curated versions of each dataset are archived for full clarity. The dataset additionally contains all metadata required to fully interpret these data, including the RGB tables used to prepare Fig 4 in Macgregor et al. (2018).

Macgregor et al. (2018) Construction, validation and application of nocturnal pollen transport networks in an agro-ecosystem: a comparison using light microscopy and DNA metabarcoding. Ecological Entomology, doi: 10.1111/een.12674.

This work was funded by the Natural Environment Research Council (grant ID: NE/K007394/1).
Files (164.5 kB)
Name Size
MicroscopyIndivs.csv
md5:768a87aae6a3881e01fe974c6763fa44
19.0 kB Download
moth_metadata.csv
md5:79340fdfc7c54ff5b8d0d48eca894a2a
12.0 kB Download
MothPollenFinal-by-taxonomy-readcounts.blast.csv
md5:bfd0ff6b18864c8716caa0fb91921e6e
46.9 kB Download
MothPollenFinal_prepared.csv
md5:a3271d5a9d550b2039c6e5b61fc0d7e2
46.1 kB Download
PlantSpecies.csv
md5:0d16af4e3c40af6300de14136139046c
15.4 kB Download
PlantSpecies_corrected.csv
md5:e8cc5797859b90c9113205d93127fdf6
15.5 kB Download
RGBmoths.txt
md5:64ef267e6664fa1f4e5fbd76abec5f9b
106 Bytes Download
RGBplants.txt
md5:bbd581367ff6365f50290069003bf5d8
285 Bytes Download
Traps.csv
md5:00715a6f0e5ed57a53706e8c565e33d8
9.2 kB Download
  • Macgregor et al. (2018) Construction, validation and application of nocturnal pollen transport networks in an agro-ecosystem: a comparison using light microscopy and DNA metabarcoding. Ecological Entomology, doi: 10.1111/een.12674.

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