Published February 18, 2022 | Version v.1.0
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bollito: a flexible pipeline for comprehensive single-cell RNA-seq analyses - Melanoma tutorial

Description

Downsampled version of the melanoma dataset originally published by Ho et al (1). The dataset is composed by cells from the 451Lu cell line. There are two samples available:

Name Description R1/R2
451LU Parental cell line 2500K_451LU_L003_R*_001.fastq.gz
451LUBR3 Vemurafenib-resistant sample treated with targeted BRAF inhibitors 500K_451LUBR3_L004_R*_001.fastq.gz


(1) Ho YJ, Anaparthy N, Molik D, et al. Single-cell RNA-seq analysis identifies markers of resistance to targeted BRAF inhibitors in melanoma cell populations. Genome Res. 2018;28(9):1353-1363. doi:10.1101/gr.234062.117

Notes

The original single-cell data can be accessed through the Gene Expression Omnibus (GEO), accession number GSE108394.

Files

Files (1.8 GB)

Name Size
md5:1a806eddf96041d300dada2da805aaa7
173.4 MB Download
md5:898f106e48740d341960f0ef898d61b1
721.3 MB Download
md5:b9f5e4de9945a517c9b756c6d255fd75
175.1 MB Download
md5:e3e51f4c8ad9ed9b7a4574d645952352
720.9 MB Download

Additional details

References

  • Ho YJ, Anaparthy N, Molik D, et al. Single-cell RNA-seq analysis identifies markers of resistance to targeted BRAF inhibitors in melanoma cell populations. Genome Res. 2018;28(9):1353-1363. doi:10.1101/gr.234062.117
  • García-Jimeno, L., Fustero-Torre, C., Jiménez-Santos, M. J., Gómez-López, G., Di Domenico, T., & Al-Shahrour, F. (2022). bollito: a flexible pipeline for comprehensive single-cell RNA-seq analyses. Bioinformatics, 38(4), 1155-1156.