THE OPIOID GROWTH FACTOR REDUCE ACTINE AND COLLAGEN EXPRESSION INDUCED BY TGF-β IN HUMAN FIBROBLASTS (IPF AND HEALTHY DONORS)

Healthy lines of fibroblasts were obtained from three primary and two commercial lines of healthy lung fibroblasts, and IPF lines were obtained from lung biopsies of four IPF patients. Both groups were studied under basal conditions and following stimulation with TGF-β. Each experiment was performed at least three times in three lines. The OGFr, Col1a1 and α-SMA expression were measured by rt-qPCR, and stress actin fibers were evaluated using immunohistochemistry (phalloidin staining). The cell growth rate was determined with the WST-1 assay.