UV mutagenesis conjugated to high throughput screening as a tool to generate new phenotypic diversity in wine yeast

The current global changes, societal and climatic, strongly challenge the wine industry. Multiple methods are applied in the development of new strains for the industry, but many are based on the existing phenotypic and genetic diversities. UV mutagenesis, as an untargeted strategy, has been successfully used for years, with significant examples on wine. Here we developed and validated a UV-mutant generation strategy coupled with a high throughput screening in wine-like conditions. This strategy led to the production of a 502 mutant’s library for which concentrations of eight primary metabolites after fermentation were assessed. This data paper presents the resulting data.