Published September 18, 2024 | Version v1
Dataset Open

Time-Resolved Epifluorescence Wide-Field Imaging of Gastruloids and Trunk-Like Structures

Description

Epifluorescence wide-field imaging of developing gastruloids and trunk-like-structures carrying a T::H2B-mCherry and Sox2::H2B-Venus reporter. Gastruloids and trunk-like-structures are mouse embryonic stem cell based embryo models (SEMs) of the embryonic trunk (Veenvliet et al., 2020). SEMs were cultured in separate wells and imaged over 4 days, every 24h. 

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Additional details

Related works

Is part of
Preprint: 10.1101/2023.12.04.569921 (DOI)

Funding

Max Planck Society
European Council for Construction Research, Development and Innovation
Horizon-EIC-2021-PathfinderChallenges-01 SUMO 101071203

Dates

Created
2024-01-11

References

  • Veenvliet JV, Bolondi A, Kretzmer H, Haut L, Scholze-Wittler M, Schifferl D, Koch F, Guignard L, Kumar AS, Pustet M, Heimann S, Buschow R, Wittler L, Timmermann B, Meissner A, Herrmann BG. Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites. Science. 2020 Dec 11;370(6522):eaba4937. doi: 10.1126/science.aba4937. PMID: 33303587.