EncyclopeDIA- Galaxy Training Network
Authors/Creators
Description
The EncyclopeDIA Searle et al. 2020 workflow described in this tutorial contains three steps. The first step is the conversion of the input data files from RAW file-types to mzML file-type using the tool msconvert. The second step which is a critical component of this workflow is the generation of a Chromatogram Library. Chromatogram Library is generated by employing the gas-phase fractionation (GPF) method and can make use of DDA-generated or predicted spectral libraries. Lastly, the experimental wide-window DIA files are analyzed using the EncyclopeDIA software tool generated by Brian Searle. EncyclopeDIA analysis of experimental DIA data against the generated Chromatogram Library and the background proteome FASTA file generates peptide and protein quantitation outputs. The availability of EncyclopeDIA workflow within the Galaxy platform offers another easier way of analyzing DIA data.
Files
LOG.txt
Files
(28.4 GB)
| Name | Size | |
|---|---|---|
|
md5:f97a7876b2d10c5cf8b652d4130fa8b4
|
1.7 GB | Download |
|
md5:317d3f6a58562e59d119e52b04051f03
|
1.7 GB | Download |
|
md5:dc8fe97a1f4f51f9af9283b475339173
|
1.0 GB | Download |
|
md5:85ad36597b93375aed6ea5737ebc52ea
|
1.2 GB | Download |
|
md5:5f44bc490426fe8512fb44f0a2761665
|
1.2 GB | Download |
|
md5:a1d9e7e33d0d7295e8cbcf4e32912b34
|
1.3 GB | Download |
|
md5:598120cbdbea7dba6d385aa3b1cfbed6
|
1.3 GB | Download |
|
md5:e3a65868de2b55a38a24982078c39467
|
1.4 GB | Download |
|
md5:345419e13dfc7bcd3b5d1e5c5fea1e0a
|
1.2 GB | Download |
|
md5:76be72595a43c0f3a211f701f52b257b
|
1.3 GB | Download |
|
md5:1654e7a0045d35e40aed095b7486aa28
|
905.8 MB | Download |
|
md5:e3f1d1a9ac9d87bc6c014d35891a8fee
|
1.1 GB | Download |
|
md5:f8af8d16e52d55490eb1dce12b85afd6
|
742.5 MB | Download |
|
md5:d404dfc28b21c95f7803b30bb0feef0f
|
989.5 MB | Download |
|
md5:360e6f0479ed7a165207b5caa3441f2f
|
1.8 GB | Download |
|
md5:073d649ed4a0fb6b133a557920c41e65
|
1.7 GB | Download |
|
md5:7794ea125674cfeb1cab5263cde3c61e
|
1.7 GB | Download |
|
md5:a2c2c5becf415cbab7bb3192435d613b
|
1.7 GB | Download |
|
md5:d44872398ae61b1e3f4d5a553fcb11d8
|
1.7 GB | Download |
|
md5:7dcaba71ced3cf0a998ec5b7bab66fa8
|
1.7 GB | Download |
|
md5:6322e218ec490324e85a68a0b1d99122
|
5.4 MB | Download |
|
md5:e8f34952b64da79330aa2d5dcea6afec
|
85.1 MB | Download |
|
md5:fa9bda3592eb64cce2355621b6ad9f6b
|
2.2 MB | Preview Download |
|
md5:44cb4c4874135429e9ef92ef98161d7b
|
2.4 MB | Download |
|
md5:9af93ac44a28c0748f8c29d8eceea7ff
|
633.1 kB | Download |
|
md5:d6b320f07e91f2850e1d414fe57308ad
|
219.0 MB | Download |
|
md5:ccf4aa41a8028803fa31488d8ae628ed
|
1.4 MB | Preview Download |
|
md5:75ff9a2d53f0637dc20d6a00ef5149db
|
724.1 MB | Download |